| pubmed-article:2161053 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0021335 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C1261468 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0017968 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0205359 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2161053 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2161053 | pubmed:dateCreated | 1990-7-3 | lld:pubmed |
| pubmed-article:2161053 | pubmed:abstractText | Bovine MDBK cells were transfected with Rous sarcoma virus-based vectors for constitutive expression of the bovine herpesvirus type 1 (BHV-1) glycoprotein, gI. Cell lines stably expressing recombinant gI were cloned and characterized. Recombinant gI was localized intracellularly, predominantly in a perinuclear region, and on the cell surface. Cells expressing gI exhibited spontaneous polykaryon formation, thus confirming the fusogenic activity described previously in gI-expressing transfected murine LMTK- cells. The recombinant form of gI synthesized in transfected MDBK cells was similar in Mr to the form expressed in BHV-1-infected MDBK cells, unlike the recombinant form of gI expressed by LMTK- cells which is deficient in N-linked glycosylation. It was concluded that cell fusion associated with the expression of BHV-1 gI in transfected mammalian cells is a reproducible phenomenon in a number of cell types and is not due to species-specific factors or expression of abnormally glycosylated gI. Cell fusion is a useful in vitro marker for gI function and may contribute to the spread of BHV-1 infections in vivo. | lld:pubmed |
| pubmed-article:2161053 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2161053 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2161053 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2161053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2161053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2161053 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2161053 | pubmed:month | May | lld:pubmed |
| pubmed-article:2161053 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:2161053 | pubmed:author | pubmed-author:BabiukL ALA | lld:pubmed |
| pubmed-article:2161053 | pubmed:author | pubmed-author:ZambT JTJ | lld:pubmed |
| pubmed-article:2161053 | pubmed:author | pubmed-author:FitzpatrickD... | lld:pubmed |
| pubmed-article:2161053 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2161053 | pubmed:volume | 71 ( Pt 5) | lld:pubmed |
| pubmed-article:2161053 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2161053 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2161053 | pubmed:pagination | 1215-9 | lld:pubmed |
| pubmed-article:2161053 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:meshHeading | pubmed-meshheading:2161053-... | lld:pubmed |
| pubmed-article:2161053 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2161053 | pubmed:articleTitle | Expression of bovine herpesvirus type 1 glycoprotein gI in transfected bovine cells induces spontaneous cell fusion. | lld:pubmed |
| pubmed-article:2161053 | pubmed:affiliation | Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada. | lld:pubmed |
| pubmed-article:2161053 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2161053 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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