| pubmed-article:21602524 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C0023434 | lld:lifeskim |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C0023690 | lld:lifeskim |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C2754998 | lld:lifeskim |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C1979963 | lld:lifeskim |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C2003903 | lld:lifeskim |
| pubmed-article:21602524 | lifeskim:mentions | umls-concept:C1710548 | lld:lifeskim |
| pubmed-article:21602524 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21602524 | pubmed:dateCreated | 2011-7-29 | lld:pubmed |
| pubmed-article:21602524 | pubmed:abstractText | Chronic lymphocytic leukemia (CLL) is characterized by a highly variable clinical course with 2 extreme subsets: indolent, ZAP70(-) and mutated immunoglobulin heavy chain gene (M-CLL); and aggressive, ZAP70(+) and unmutated immunoglobulin heavy chain (UM-CLL). Given the long-term suspicion of antigenic stimulation as a primum movens in the disease, the role of the B-cell receptor has been extensively studied in various experimental settings; albeit scarcely in a comparative dynamic proteomic approach. Here we use a quantitative 2-dimensional fluorescence difference gel electrophoresis technology to compare 48 proteomic profiles of the 2 CLL subsets before and after anti-IgM ligation. Differentially expressed proteins were subsequently identified by mass spectrometry. We show that unstimulated M- and UM-CLL cells display distinct proteomic profiles. Furthermore, anti-IgM stimulation induces a specific proteomic response, more pronounced in the more aggressive CLL. Statistical analyses demonstrate several significant protein variations according to stimulation conditions. Finally, we identify an intermediate form of M-CLL cells, with an indolent profile (ZAP70(-)) but sharing aggressive proteomic profiles alike UM-CLL cells. Collectively, this first quantitative and dynamic proteome analysis of CLL further dissects the complex molecular pathway after B-cell receptor stimulation and depicts distinct proteomic profiles, which could lead to novel molecular stratification of the disease. | lld:pubmed |
| pubmed-article:21602524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21602524 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:21602524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21602524 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21602524 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:21602524 | pubmed:issn | 1528-0020 | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:LeblondVéroni... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:HerbrechtRaou... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:BahramSeiamak... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:GribbenJohn... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:BénéMarie-Chr... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:Merle-BéralHé... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:DaviFrédéricF | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:JacobFrédéric... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:VallatLaurent... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:NadaudSophieS | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:PionneauCédri... | lld:pubmed |
| pubmed-article:21602524 | pubmed:author | pubmed-author:PerrotAuroreA | lld:pubmed |
| pubmed-article:21602524 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21602524 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:21602524 | pubmed:volume | 118 | lld:pubmed |
| pubmed-article:21602524 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21602524 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21602524 | pubmed:pagination | e1-15 | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:meshHeading | pubmed-meshheading:21602524... | lld:pubmed |
| pubmed-article:21602524 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21602524 | pubmed:articleTitle | A unique proteomic profile on surface IgM ligation in unmutated chronic lymphocytic leukemia. | lld:pubmed |
| pubmed-article:21602524 | pubmed:affiliation | Laboratoire d'Immunogénétique Moléculaire Humaine, Centre de Recherche d'Immunologie et d'Hématologie, Faculté de Médecine, Université de Strasbourg, Strasbourg, France. | lld:pubmed |
| pubmed-article:21602524 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21602524 | pubmed:publicationType | Comment | lld:pubmed |
| pubmed-article:21602524 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:21602524 | lld:pubmed |
