| pubmed-article:2158096 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2158096 | lifeskim:mentions | umls-concept:C1721094 | lld:lifeskim |
| pubmed-article:2158096 | lifeskim:mentions | umls-concept:C1335945 | lld:lifeskim |
| pubmed-article:2158096 | lifeskim:mentions | umls-concept:C0282642 | lld:lifeskim |
| pubmed-article:2158096 | lifeskim:mentions | umls-concept:C0450442 | lld:lifeskim |
| pubmed-article:2158096 | lifeskim:mentions | umls-concept:C0439836 | lld:lifeskim |
| pubmed-article:2158096 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2158096 | pubmed:dateCreated | 1990-5-24 | lld:pubmed |
| pubmed-article:2158096 | pubmed:abstractText | Escherichia coli catabolite gene activator protein (CAP) is a helix-turn-helix motif sequence-specific DNA binding protein [de Crombrugghe, B., Busby, S. & Buc, H. (1984) Science 224, 831-838; and Pabo, C. & Sauer, R. (1984) Annu. Rev. Biochem. 53, 293-321]. In this work, CAP has been converted into a site-specific DNA cleavage agent by incorporation of the chelator 1,10-phenanthroline at amino acid 10 of the helix-turn-helix motif. [(N-Acetyl-5-amino-1,10-phenanthroline)-Cys178]CAP binds to a 22-base-pair DNA recognition site with Kobs = 1 x 10(8) M-1. In the presence of Cu(II) and reducing agent, [(N-acetyl-5-amino-1,10-phenanthroline)-Cys178]CAP cleaves DNA at four adjacent nucleotides on each DNA strand within the DNA recognition site. The DNA cleavage reaction has been demonstrated using 40-base-pair and 7164-base-pair DNA substrates. The DNA cleavage reaction is not inhibited by dam methylation of the DNA substrate. Such semisynthetic site-specific DNA cleavage agents have potential applications in chromosome mapping, cloning, and sequencing. | lld:pubmed |
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| pubmed-article:2158096 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2158096 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2158096 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2158096 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2158096 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2158096 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:2158096 | pubmed:author | pubmed-author:EbrightR HRH | lld:pubmed |
| pubmed-article:2158096 | pubmed:author | pubmed-author:EbrightY WYW | lld:pubmed |
| pubmed-article:2158096 | pubmed:author | pubmed-author:GunasekeraAA | lld:pubmed |
| pubmed-article:2158096 | pubmed:author | pubmed-author:PendergrastP... | lld:pubmed |
| pubmed-article:2158096 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2158096 | pubmed:volume | 87 | lld:pubmed |
| pubmed-article:2158096 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2158096 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2158096 | pubmed:pagination | 2882-6 | lld:pubmed |
| pubmed-article:2158096 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2158096 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2158096 | pubmed:articleTitle | Conversion of a helix-turn-helix motif sequence-specific DNA binding protein into a site-specific DNA cleavage agent. | lld:pubmed |
| pubmed-article:2158096 | pubmed:affiliation | Department of Chemistry, Rutgers University, New Brunswick, NJ 08855. | lld:pubmed |
| pubmed-article:2158096 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2158096 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2158096 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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