| pubmed-article:2157066 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2157066 | lifeskim:mentions | umls-concept:C0014662 | lld:lifeskim |
| pubmed-article:2157066 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:2157066 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:2157066 | lifeskim:mentions | umls-concept:C0449774 | lld:lifeskim |
| pubmed-article:2157066 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:2157066 | pubmed:dateCreated | 1990-5-3 | lld:pubmed |
| pubmed-article:2157066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2157066 | pubmed:abstractText | The pattern of expression of the equine infectious anemia virus (EIAV) genome in a persistently infected canine cell line was determined. Five EIAV-specific transcripts (8.2, 5.0, 4.0, 2, and 1.8 kilobases [kb]) were detected by using subgenomic restriction enzyme fragments of EIAV DNA and EIAV-specific oligonucleotides as probes. The 8.2-kb mRNA could be shown to represent viral genomic RNA, whereas the smaller transcripts were generated by splicing events. Evidence was obtained that indicated that each subgenomic RNA species shared a common 5'-splice donor. The 5.0-kb mRNA was found to be expressed at relatively low levels, was difficult to detect consistently, and appeared to be generated by a single splicing event which linked the 5' exon to the 3' region of pol. The 4.0-kb transcript was concluded to be the env mRNA on the basis of its hybridization pattern with the various probes and its abundance. The 2-kb species was found to be multiply spliced and was encoded by sequences derived from orf2 but was not detected by probes representing 3'-env/3'-orf sequences. The 1.8-kb species was shown to consist of sequences representing orf1, part of orf2, and the 3'-orf/env and may represent the message for the EIAV trans-activator gene. | lld:pubmed |
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| pubmed-article:2157066 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2157066 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2157066 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2157066 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2157066 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2157066 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:2157066 | pubmed:author | pubmed-author:ShermanLL | lld:pubmed |
| pubmed-article:2157066 | pubmed:author | pubmed-author:TronickS RSR | lld:pubmed |
| pubmed-article:2157066 | pubmed:author | pubmed-author:YanivAA | lld:pubmed |
| pubmed-article:2157066 | pubmed:author | pubmed-author:GazitAA | lld:pubmed |
| pubmed-article:2157066 | pubmed:author | pubmed-author:NoimanSS | lld:pubmed |
| pubmed-article:2157066 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2157066 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:2157066 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2157066 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2157066 | pubmed:pagination | 1839-43 | lld:pubmed |
| pubmed-article:2157066 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2157066 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2157066 | pubmed:articleTitle | Pattern of transcription of the genome of equine infectious anemia virus. | lld:pubmed |
| pubmed-article:2157066 | pubmed:affiliation | Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel. | lld:pubmed |
| pubmed-article:2157066 | pubmed:publicationType | Journal Article | lld:pubmed |
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