| pubmed-article:2156621 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0149925 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0431085 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0443224 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0596138 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:2156621 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:2156621 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2156621 | pubmed:dateCreated | 1990-5-3 | lld:pubmed |
| pubmed-article:2156621 | pubmed:abstractText | We showed previously that insulin-like growth factor I (IGF-I) is detectable in small cell lung cancer (SCLC) tumor biopsies and cell lines and that recombinant human IGF-I stimulates DNA synthesis in SCLC cells. Here we report further studies on the role of IGF-I in 2 SCLC cell lines: HC12, classic; and ICR-SC17, variant. Immunoreactive IGF-I was detected in medium conditioned by HC12 but not ICR-SC17. Both HC12 and ICR-SC17 bound IGF-I with 100-fold greater affinity than insulin. Scatchard analysis revealed two classes of IGF-I binding site of high (Kd 0.1 nM, n = 2,300) and lower (Kd 3 nM, n = 28,000) affinity. In both cell lines [3H]thymidine incorporation was enhanced by recombinant human IGF-I, 100-1000 ng/ml. ICR-SC17 also showed growth enhancement as measured by increase in cell numbers. There was no response in HC12, probably due to endogenous IGF-I production. 125I-IGF-I binding and basal and IGF-I-stimulated mitogenesis were inhibited by monoclonal antibodies to IGF-I (SM1.20B, SM1.25) or the type I IGF receptor alpha IR3 but not an isotypic control monoclonal antibody. Antiproliferative effects were manifest in [3H]thymidine incorporation assays in serum-free conditions and growth of serum-supplemented liquid cultures. We also tested fresh or newly cultured tumor cells obtained by fine needle aspiration of metastases in three previously untreated and four relapsed patients with SCLC. IGF-I binding sites were demonstrable on fresh SCLC cells, and specific binding was inhibited by SM1.20B. All seven samples showed stimulation of [3H]thymidine incorporation in the presence of recombinant human IGF-I, 100-500 ng/ml. As in cultured cells, basal and IGF-I-stimulated DNA synthesis was inhibited by monoclonal antibodies SM1.20B, SM1.25, and alpha IR3 but not the isotypic control. These results confirm the findings of previous studies and suggest that IGF-I can function as an autocrine growth factor in SCLC in vitro and possibly also in vivo. | lld:pubmed |
| pubmed-article:2156621 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2156621 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2156621 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2156621 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2156621 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2156621 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:SmithI EIE | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:TealeJ DJD | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:Van WykJ JJJ | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:MillarJ LJL | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:TrottP APA | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:MacaulayV MVM | lld:pubmed |
| pubmed-article:2156621 | pubmed:author | pubmed-author:EverardM JMJ | lld:pubmed |
| pubmed-article:2156621 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2156621 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2156621 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:2156621 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2156621 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2156621 | pubmed:pagination | 2511-7 | lld:pubmed |
| pubmed-article:2156621 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:2156621 | pubmed:meshHeading | pubmed-meshheading:2156621-... | lld:pubmed |
| pubmed-article:2156621 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2156621 | pubmed:articleTitle | Autocrine function for insulin-like growth factor I in human small cell lung cancer cell lines and fresh tumor cells. | lld:pubmed |
| pubmed-article:2156621 | pubmed:affiliation | Section of Medicine Research Laboratories, Institute of Cancer Research, Belmont, Surrey, United Kingdom. | lld:pubmed |
| pubmed-article:2156621 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2156621 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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