| pubmed-article:21539388 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21539388 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:21539388 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:21539388 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:21539388 | pubmed:dateCreated | 2011-6-15 | lld:pubmed |
| pubmed-article:21539388 | pubmed:abstractText | 2'-O-[(2-Bromoethoxy)methyl]cytidine and 2'-O-[(2-azidoethoxy)methyl]cytidine have been prepared and introduced as appropriately protected 3'-phosphoramidite (1) and 3'-(H-phosphonate) (2) building blocks, respectively, into 2'-O-methyl oligoribonucleotides. The support-bound oligonucleotides were subjected to two consecutive conjugations with alkynyl-functionalized monosaccharides. The first saccharide was introduced by a Cu(I) promoted click reaction with 2 and the second by azidation of the 2-bromoethoxy group of 1 followed by the click reaction. The influence of the 2'-glycoconjugations on hybridization with DNA and 2'-O-methyl RNA targets was studied. Two saccharide units within a 15-mer oligonucleotide had a barely noticeable effect on the duplex stability, while introduction of a third one moderately decreased the melting temperature. | lld:pubmed |
| pubmed-article:21539388 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21539388 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21539388 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21539388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21539388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21539388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21539388 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21539388 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21539388 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:21539388 | pubmed:issn | 1520-4812 | lld:pubmed |
| pubmed-article:21539388 | pubmed:author | pubmed-author:LönnbergHarri... | lld:pubmed |
| pubmed-article:21539388 | pubmed:author | pubmed-author:MikhailovSerg... | lld:pubmed |
| pubmed-article:21539388 | pubmed:author | pubmed-author:VirtaPasiP | lld:pubmed |
| pubmed-article:21539388 | pubmed:author | pubmed-author:KiviniemiAnuA | lld:pubmed |
| pubmed-article:21539388 | pubmed:author | pubmed-author:DrenichevMikh... | lld:pubmed |
| pubmed-article:21539388 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21539388 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:21539388 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:21539388 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21539388 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21539388 | pubmed:pagination | 1249-55 | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:meshHeading | pubmed-meshheading:21539388... | lld:pubmed |
| pubmed-article:21539388 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21539388 | pubmed:articleTitle | Solid-supported 2'-O-glycoconjugation of oligonucleotides by azidation and click reactions. | lld:pubmed |
| pubmed-article:21539388 | pubmed:affiliation | Department of Chemistry, University of Turku, FIN-20014 Turku, Finland. anu.kiviniemi@utu.fi | lld:pubmed |
| pubmed-article:21539388 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21539388 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
