| pubmed-article:21533184 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21533184 | lifeskim:mentions | umls-concept:C0040160 | lld:lifeskim |
| pubmed-article:21533184 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:21533184 | lifeskim:mentions | umls-concept:C0162493 | lld:lifeskim |
| pubmed-article:21533184 | lifeskim:mentions | umls-concept:C1414993 | lld:lifeskim |
| pubmed-article:21533184 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:21533184 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21533184 | pubmed:dateCreated | 2011-5-2 | lld:pubmed |
| pubmed-article:21533184 | pubmed:abstractText | Thyrotropin-releasing hormone (TRH) activates not only the secretion of thyrotropin (TSH) but also the transcription of TSH? and ?-glycoprotein (?GSU) subunit genes. TSH? expression is maintained by two transcription factors, Pit1 and GATA2, and is negatively regulated by thyroid hormone (T3). Our prior studies suggest that the main activator of the TSH? gene is GATA2, not Pit1 or unliganded T3 receptor (TR). In previous studies on the mechanism of TRH-induced activation of the TSH? gene, the involvements of Pit1 and TR have been investigated, but the role of GATA2 has not been clarified. Using kidney-derived CV1 cells and pituitary-derived GH3 and T?T1 cells, we demonstrate here that TRH signaling enhances GATA2-dependent activation of the TSH? promoter and that TRH-induced activity is abolished by amino acid substitution in the GATA2-Zn finger domain or mutation of GATA-responsive element in the TSH? gene. In CV1 cells transfected with TRH receptor expression plasmid, GATA2-dependent transactivation of ?GSU and endothelin-1 promoters was enhanced by TRH. In the gel shift assay, TRH signal potentiated the DNA-binding capacity of GATA2. While inhibition by T3 is dominant over TRH-induced activation, unliganded TR or the putative negative T3-responsive element are not required for TRH-induced stimulation. Studies using GH3 cells showed that TRH-induced activity of the TSH? promoter depends on protein kinase C but not the mitogen-activated protein kinase, suggesting that the signaling pathway is different from that in the prolactin gene. These results indicate that GATA2 is the principal mediator of the TRH signaling pathway in TSH? expression. | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21533184 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21533184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21533184 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21533184 | pubmed:issn | 1932-6203 | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:MatsushitaAki... | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:NakamuraHirot... | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:SuzukiShingoS | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:MatsunagaHide... | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:OkiYutakaY | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:IwakiHiroyuki... | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:OhbaKenjiK | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:SasakiShigeka... | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:MisawaHirokoH | lld:pubmed |
| pubmed-article:21533184 | pubmed:author | pubmed-author:IshizukaKeiko... | lld:pubmed |
| pubmed-article:21533184 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21533184 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:21533184 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21533184 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21533184 | pubmed:pagination | e18667 | lld:pubmed |
| pubmed-article:21533184 | pubmed:meshHeading | pubmed-meshheading:21533184... | lld:pubmed |
| pubmed-article:21533184 | pubmed:meshHeading | pubmed-meshheading:21533184... | lld:pubmed |
| pubmed-article:21533184 | pubmed:meshHeading | pubmed-meshheading:21533184... | lld:pubmed |
