| pubmed-article:21515401 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21515401 | lifeskim:mentions | umls-concept:C0446021 | lld:lifeskim |
| pubmed-article:21515401 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21515401 | pubmed:dateCreated | 2011-6-13 | lld:pubmed |
| pubmed-article:21515401 | pubmed:abstractText | d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 ± 2g d-xylonatel(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 gd-galactosel(-1) and 40 g d-xylosel(-1). Intracellular accumulation of d-xylonate (up to ?70 mg [gbiomass](-1)) was observed. d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO(2)l(-1)h(-1) without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12 mmolO(2)l(-1)h(-1) were provided. d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1?XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1?XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2. | lld:pubmed |
| pubmed-article:21515401 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21515401 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21515401 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21515401 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:21515401 | pubmed:issn | 1096-7184 | lld:pubmed |
| pubmed-article:21515401 | pubmed:author | pubmed-author:PenttiläMerja... | lld:pubmed |
| pubmed-article:21515401 | pubmed:author | pubmed-author:RuohonenLaura... | lld:pubmed |
| pubmed-article:21515401 | pubmed:author | pubmed-author:WiebeMarilyn... | lld:pubmed |
| pubmed-article:21515401 | pubmed:author | pubmed-author:ToivariMervi... | lld:pubmed |
| pubmed-article:21515401 | pubmed:author | pubmed-author:NygårdYvonneY | lld:pubmed |
| pubmed-article:21515401 | pubmed:copyrightInfo | Copyright © 2011 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:21515401 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21515401 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:21515401 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21515401 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21515401 | pubmed:pagination | 383-91 | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:meshHeading | pubmed-meshheading:21515401... | lld:pubmed |
| pubmed-article:21515401 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21515401 | pubmed:articleTitle | Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis. | lld:pubmed |
| pubmed-article:21515401 | pubmed:affiliation | VTT, Technical Research Centre of Finland, VTT Espoo, Finland. yvonne.nygard@vtt.fi | lld:pubmed |
| pubmed-article:21515401 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21515401 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
