| pubmed-article:21494745 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0086247 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0014707 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0001992 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0233820 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0596040 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0034917 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0007382 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C1157156 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0439836 | lld:lifeskim |
| pubmed-article:21494745 | lifeskim:mentions | umls-concept:C0050993 | lld:lifeskim |
| pubmed-article:21494745 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:21494745 | pubmed:dateCreated | 2011-5-19 | lld:pubmed |
| pubmed-article:21494745 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:abstractText | Ergot alkaloids are indole derivatives with diverse structures and biological activities. They are produced by a wide range of fungi with Claviceps purpurea as the most important producer for medical use. Chanoclavine-I aldehyde is proposed as a branch point via festuclavine or pyroclavine to clavine-type alkaloids in Trichocomaceae and via agroclavine to ergoamides and ergopeptines in Clavicipitaceae. Here we report the conversion of chanoclavine-I aldehyde to agroclavine by EasG from Claviceps purpurea, a homologue of the festuclavine synthase FgaFS in Aspergillus fumigatus, in the presence of reduced glutathione and NADPH. EasG comprises 290 amino acids with a molecular mass of about 31.9 kDa. The soluble monomeric His(6)-EasG was purified after overproduction in E. coli by affinity chromatography and used for enzyme assays. The structure of agroclavine was unequivocally elucidated by NMR and MS analyses. | lld:pubmed |
| pubmed-article:21494745 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21494745 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21494745 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21494745 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:21494745 | pubmed:issn | 1477-0539 | lld:pubmed |
| pubmed-article:21494745 | pubmed:author | pubmed-author:LiShu-MingSM | lld:pubmed |
| pubmed-article:21494745 | pubmed:author | pubmed-author:XieXiulanX | lld:pubmed |
| pubmed-article:21494745 | pubmed:author | pubmed-author:WallweyChrist... | lld:pubmed |
| pubmed-article:21494745 | pubmed:author | pubmed-author:MatuschekMarc... | lld:pubmed |
| pubmed-article:21494745 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21494745 | pubmed:day | 7 | lld:pubmed |
| pubmed-article:21494745 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:21494745 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21494745 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21494745 | pubmed:pagination | 4328-35 | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:meshHeading | pubmed-meshheading:21494745... | lld:pubmed |
| pubmed-article:21494745 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21494745 | pubmed:articleTitle | New insights into ergot alkaloid biosynthesis in Claviceps purpurea: an agroclavine synthase EasG catalyses, via a non-enzymatic adduct with reduced glutathione, the conversion of chanoclavine-I aldehyde to agroclavine. | lld:pubmed |
| pubmed-article:21494745 | pubmed:affiliation | Philipps-Universität Marburg, Institut für Pharmazeutische Biologie und Biotechnologie, Deutschhausstrasse 17A, D-35037, Marburg, Germany. | lld:pubmed |
| pubmed-article:21494745 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21494745 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
