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pubmed-article:21481235pubmed:abstractTextNumerous microRNAs (miRNAs) have heterogeneous ends resulting from imprecise cleavages by processing nucleases and from various non-templated nucleotide additions. The scale of miRNA end-heterogeneity is best shown by deep sequencing data revealing not only the major miRNA variants but also those that occur in only minute amounts and are unlikely to be of functional importance. All RNA interference (RNAi) technology reagents that are expressed and processed in cells are also exposed to the same machinery generating end-heterogeneity of the released short interfering RNAs (siRNAs) or miRNA mimetics.lld:pubmed
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pubmed-article:21481235pubmed:articleTitleNorthern blotting analysis of microRNAs, their precursors and RNA interference triggers.lld:pubmed
pubmed-article:21481235pubmed:affiliationLaboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14 Str,, 61-704 Poznan, Poland.lld:pubmed
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