| pubmed-article:21463533 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21463533 | lifeskim:mentions | umls-concept:C0996995 | lld:lifeskim |
| pubmed-article:21463533 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:21463533 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:21463533 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:21463533 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:21463533 | pubmed:dateCreated | 2011-4-21 | lld:pubmed |
| pubmed-article:21463533 | pubmed:abstractText | BACKGROUND: We have previously reported on the variation of total fructooligosaccharides (FOS), total inulooligosaccharides (IOS) and inulin in the roots of burdock stored at different temperatures. During storage at 0°C, an increase of FOS as a result of the hydrolysis of inulin was observed. Moreover, we suggested that an increase of IOS would likely be due to the synthesis of the IOS by fructosyltransfer from 1-kestose to accumulated fructose and elongated fructose oligomers which can act as acceptors for fructan:fructan 1-fructosyltransferase (1-FFT). However, enzymes such as inulinase or fructan 1-exohydorolase (1-FEH) involved in inulin degradation in burdock roots are still not known. Here, we report the isolation and functional analysis of a gene encoding burdock 1-FEH. RESULTS: A cDNA, named aleh1, was obtained by the RACE method following PCR with degenerate primers designed based on amino-acid sequences of FEHs from other plants. The aleh1 encoded a polypeptide of 581 amino acids. The relative molecular mass and isoelectric point (pI) of the deduced polypeptide were calculated to be 65,666 and 4.86. A recombinant protein of aleh1 was produced in Pichia pastoris, and was purified by ion exchange chromatography with DEAE-Sepharose CL-6B, hydrophobic chromatography with Toyopearl HW55S and gel filtration chromatography with Toyopearl HW55S. Purified recombinant protein showed hydrolyzing activity against ?-2, 1 type fructans such as 1-kestose, nystose, fructosylnystose and inulin. On the other hand, sucrose, neokestose, 6-kestose and high DP levan were poor substrates.The purified recombinant protein released fructose from sugars extracted from burdock roots. These results indicated that aleh1 encoded 1-FEH. | lld:pubmed |
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| pubmed-article:21463533 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21463533 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21463533 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:21463533 | pubmed:issn | 1752-153X | lld:pubmed |
| pubmed-article:21463533 | pubmed:author | pubmed-author:YoshidaMidori... | lld:pubmed |
| pubmed-article:21463533 | pubmed:author | pubmed-author:OnoderaShuich... | lld:pubmed |
| pubmed-article:21463533 | pubmed:author | pubmed-author:ShiomiNorioN | lld:pubmed |
| pubmed-article:21463533 | pubmed:author | pubmed-author:UenoKeijiK | lld:pubmed |
| pubmed-article:21463533 | pubmed:author | pubmed-author:IshiguroYojir... | lld:pubmed |
| pubmed-article:21463533 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21463533 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:21463533 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21463533 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21463533 | pubmed:pagination | 16 | lld:pubmed |
| pubmed-article:21463533 | pubmed:dateRevised | 2011-9-2 | lld:pubmed |
| pubmed-article:21463533 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21463533 | pubmed:articleTitle | Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.). | lld:pubmed |
| pubmed-article:21463533 | pubmed:affiliation | Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, 582 Bunkyodai Midorimachi, Ebetsu, 069-8501, Japan. n-shiomi@rakuno.ac.jp. | lld:pubmed |
| pubmed-article:21463533 | pubmed:publicationType | Journal Article | lld:pubmed |
