| pubmed-article:21457753 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0002570 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C1416402 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C1416891 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0205266 | lld:lifeskim |
| pubmed-article:21457753 | lifeskim:mentions | umls-concept:C0291751 | lld:lifeskim |
| pubmed-article:21457753 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:21457753 | pubmed:dateCreated | 2011-6-10 | lld:pubmed |
| pubmed-article:21457753 | pubmed:abstractText | Insulin regulated aminopeptidase (IRAP) recognises "AT(4)-receptor" ligands like angiotensin IV (Ang IV) and peptidomimetics like AL-11. The metabolic stability and high affinity of [(3)H]AL-11 for catalytically active IRAP allowed its detection in Chinese hamster ovary (CHO-K1) cell membranes in the absence of chelators (Demaegdt et al., 2009). Here, we show that, contrary to [(3)H]Ang IV, [(3)H]AL-11 displays high affinity and specificity for IRAP in intact CHO-K1 cells as well. After binding to IRAP at the surface, [(3)H]AL-11 is effectively internalized by an endocytotic process. Unexpectedly, surface binding and internalization of [(3)H]AL-11 was not affected by pretreating the cells with Ang IV but declined with AL-11. In the latter case surface expression of IRAP even increased. After elimination of simpler explanations, it is proposed that metabolically stable "AT(4)-receptor" ligands undergo semi-continuous cycling between the cell surface and endosomal compartments. The in vivo efficacy of stable and unstable "AT(4)-receptor" ligands could therefore differ. | lld:pubmed |
| pubmed-article:21457753 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21457753 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21457753 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21457753 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:21457753 | pubmed:issn | 1872-8057 | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:TourwéDirkD | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:VauquelinGeor... | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:TóthGézaG | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:GardPaulP | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:De... | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:DemaegdtHeidi... | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:SzemenyeiErzs... | lld:pubmed |
| pubmed-article:21457753 | pubmed:author | pubmed-author:LukaszukAneta... | lld:pubmed |
| pubmed-article:21457753 | pubmed:copyrightInfo | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:21457753 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21457753 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:21457753 | pubmed:volume | 339 | lld:pubmed |
| pubmed-article:21457753 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21457753 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21457753 | pubmed:pagination | 34-44 | lld:pubmed |
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| pubmed-article:21457753 | pubmed:meshHeading | pubmed-meshheading:21457753... | lld:pubmed |
| pubmed-article:21457753 | pubmed:meshHeading | pubmed-meshheading:21457753... | lld:pubmed |
| pubmed-article:21457753 | pubmed:meshHeading | pubmed-meshheading:21457753... | lld:pubmed |
| pubmed-article:21457753 | pubmed:meshHeading | pubmed-meshheading:21457753... | lld:pubmed |
| pubmed-article:21457753 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21457753 | pubmed:articleTitle | Binding of "AT4 receptor" ligands to insulin regulated aminopeptidase (IRAP) in intact Chinese hamster ovary cells. | lld:pubmed |
| pubmed-article:21457753 | pubmed:affiliation | Department of Molecular and Biochemical Pharmacology, Research Group of Experimental Pharmacology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium. | lld:pubmed |
| pubmed-article:21457753 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21457753 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
