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pubmed-article:2145247pubmed:abstractTextCombinations of peripheral blood lymphocytes, matched or mismatched for HLA-DP, were analyzed in primary one-way mixed lymphocyte culture experiments. Proliferative responses as correlated with tritiated thymidine uptake were assessed over a kinetic range of 5-15 days. A proliferative response was observed between DP-mismatched combinations, whereas combinations matched for DP and all other HLA alloantigens did not elicit significant proliferation. Optimal responses were observed 9 days after the combination of 1 x 10(5) responder and stimulator cells. Responses were blocked by anti-DP monoclonal antibodies. These studies demonstrate the complexity of the primary mixed lymphocyte culture system and suggest that DP alloantigens should be considered when anomalous responses are obtained.lld:pubmed
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pubmed-article:2145247pubmed:authorpubmed-author:EckelsD DDDlld:pubmed
pubmed-article:2145247pubmed:authorpubmed-author:SellT WTWlld:pubmed
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pubmed-article:2145247pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2145247pubmed:year1990lld:pubmed
pubmed-article:2145247pubmed:articleTitlePrimary mixed lymphocyte responses to HLA-DP.lld:pubmed
pubmed-article:2145247pubmed:affiliationImmunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233.lld:pubmed
pubmed-article:2145247pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2145247pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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