| pubmed-article:2143187 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0062503 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0030518 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:2143187 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:2143187 | pubmed:issue | 23 | lld:pubmed |
| pubmed-article:2143187 | pubmed:dateCreated | 1990-9-13 | lld:pubmed |
| pubmed-article:2143187 | pubmed:abstractText | The regulation of the cellular distribution of proteoglycans in a clonal rat parathyroid cell line by extracellular Ca2+ concentrations ([Ca2+]e) was studied. Proteoglycans synthesized by the cells metabolically labeled with [35S]sulfate have been shown to be almost exclusively heparan sulfate (HS) proteoglycans (Yanagishita, M., Brandi, M.L., and Sakaguchi, K. (1989) J. Biol. Chem. 264, 15714-15720), which are generally associated with the plasma membrane. The proportion of HS proteoglycans on the cell surface was approximately 20% in 2.1 mM (high) [Ca2+]e, whereas it increased to 50-60% in 0.05 mM (low) [Ca2+]e. Cell-associated HS proteoglycans redistribute in response to changing [Ca2+]e with a t 1/2 less than 4 min; HS proteoglycans appear on the cell surface as [Ca2+]e decreases and disappear from the cell surface as [Ca2+]e increases. Further, HS proteoglycans on the cell surface recycle to and from an intracellular compartment approximately 10 times before their degradation in low [Ca2+]e but do not recycle in high [Ca2+]e. The distribution of newly synthesized HS proteoglycans is regulated by [Ca2+]e but is independent of [Ca2+]e during biosynthesis. In low [Ca2+]e, at least 50% of the HS proteoglycans pulse-labeled for 10 min are transported from the Golgi complex to the cell surface or to the recycling compartment with a t 1/2 of approximately 20 min. Another approximately 10% appear on the cell surface in either low or high [Ca2+]e in a compartment with a long half-life. Addition of Mg2+ or Ba2+ to the low [Ca2+]e cultures had little effect on the distribution of HS proteoglycans. These observations suggest that [Ca2+]e specifically regulates the distribution and recycling of cell-associated HS proteoglycans in the parathyroid cells. | lld:pubmed |
| pubmed-article:2143187 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2143187 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2143187 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2143187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2143187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2143187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2143187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2143187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2143187 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2143187 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2143187 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2143187 | pubmed:author | pubmed-author:SakaguchiKK | lld:pubmed |
| pubmed-article:2143187 | pubmed:author | pubmed-author:AurbachG DGD | lld:pubmed |
| pubmed-article:2143187 | pubmed:author | pubmed-author:TakeuchiYY | lld:pubmed |
| pubmed-article:2143187 | pubmed:author | pubmed-author:HascallV CVC | lld:pubmed |
| pubmed-article:2143187 | pubmed:author | pubmed-author:YanagishitaMM | lld:pubmed |
| pubmed-article:2143187 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2143187 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2143187 | pubmed:volume | 265 | lld:pubmed |
| pubmed-article:2143187 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2143187 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2143187 | pubmed:pagination | 13661-8 | lld:pubmed |
| pubmed-article:2143187 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:2143187 | pubmed:meshHeading | pubmed-meshheading:2143187-... | lld:pubmed |
| pubmed-article:2143187 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2143187 | pubmed:articleTitle | Extracellular calcium regulates distribution and transport of heparan sulfate proteoglycans in a rat parathyroid cell line. | lld:pubmed |
| pubmed-article:2143187 | pubmed:affiliation | Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892. | lld:pubmed |
| pubmed-article:2143187 | pubmed:publicationType | Journal Article | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2143187 | lld:pubmed |
