| pubmed-article:21427056 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21427056 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:21427056 | lifeskim:mentions | umls-concept:C0004391 | lld:lifeskim |
| pubmed-article:21427056 | lifeskim:mentions | umls-concept:C0007587 | lld:lifeskim |
| pubmed-article:21427056 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:21427056 | pubmed:dateCreated | 2011-5-23 | lld:pubmed |
| pubmed-article:21427056 | pubmed:abstractText | Aminochrome, the precursor of neuromelanin, has been proposed to be involved in the neurodegeneration neuromelanin-containing dopaminergic neurons in Parkinson's disease. We aimed to study the mechanism of aminochrome-dependent cell death in a cell line derived from rat substantia nigra. We found that aminochrome (50?M), in the presence of NAD(P)H-quinone oxidoreductase, EC 1.6.99.2 (DT)-diaphorase inhibitor dicoumarol (DIC) (100?M), induces significant cell death (62 ± 3%; p < 0.01), increase in caspase-3 activation (p < 0.001), release of cytochrome C, disruption of mitochondrial membrane potential (p < 0.01), damage of mitochondrial DNA, damage of mitochondria determined with transmission electron microscopy, a dramatic morphological change characterized as cell shrinkage, and significant increase in number of autophagic vacuoles. To determine the role of autophagy on aminochrome-induced cell death, we incubated the cells in the presence of vinblastine and rapamycin. Interestingly, 10?M vinblastine induces a 5.9-fold (p < 0.001) and twofold (p < 0.01) significant increase in cell death when the cells were incubated with 30?M aminochrome in the absence and presence of DIC, respectively, whereas 10?M rapamycin preincubated 24 h before addition of 50?M aminochrome in the absence and the presence of 100?M DIC induces a significant decrease (p < 0.001) in cell death. In conclusion, autophagy seems to be an important protective mechanism against two different aminochrome-induced cell deaths that initially showed apoptotic features. The cell death induced by aminochrome when DT-diaphorase is inhibited requires activation of mitochondrial pathway, whereas the cell death induced by aminochrome alone requires inhibition of autophagy-dependent degrading of damaged organelles and recycling through lysosomes. | lld:pubmed |
| pubmed-article:21427056 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21427056 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21427056 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21427056 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:21427056 | pubmed:issn | 1096-0929 | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:MuñozPatricia... | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:CaviedesPablo... | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:Segura-Aguila... | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:CouveEduardoE | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:ParisIrmgardI | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:SandersLaurie... | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:GreenamyreJoh... | lld:pubmed |
| pubmed-article:21427056 | pubmed:author | pubmed-author:HuenchugualaS... | lld:pubmed |
| pubmed-article:21427056 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21427056 | pubmed:volume | 121 | lld:pubmed |
| pubmed-article:21427056 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21427056 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21427056 | pubmed:pagination | 376-88 | lld:pubmed |
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| pubmed-article:21427056 | pubmed:meshHeading | pubmed-meshheading:21427056... | lld:pubmed |
| pubmed-article:21427056 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21427056 | pubmed:articleTitle | Autophagy protects against aminochrome-induced cell death in substantia nigra-derived cell line. | lld:pubmed |
| pubmed-article:21427056 | pubmed:affiliation | Program of Molecular and Clinical Pharmacology, Biomedical Science Institute, Faculty of Medicine, University of Chile, Santiago 8380453, Chile. | lld:pubmed |
| pubmed-article:21427056 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21427056 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21427056 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
