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pubmed-article:21356599pubmed:dateCreated2011-6-3lld:pubmed
pubmed-article:21356599pubmed:abstractTextThe psb30 (ycf12) gene is conserved in a wide variety of oxygenic-photosynthetic organisms except angiosperms and some marine cyanobacteria. Psb30 protein is found in cyanobacterial photosystem II (PSII) core complexes and is dispensable for PSII structure and function. The most recent three-dimensional structure of cyanobacterial PSII core complex has revealed that Psb30 is located in proximity of PsbJ, PsbK, and PsbZ. However Psb30 has not yet been detected in PSII complexes from eukaryotic photosynthetic organisms. Here we found the expression of the chloroplast psb30 gene in the green alga Chlamydomonas reinhardtii by immunoblotting and Psb30 is exclusively co-purifies with PSII core complex and is significantly reduced in PSII-deficient mutants. Partial disintegration of PSII core complex and subsequent fractionation of the resulting subcomplexes revealed that Psb30 is exclusively associated with PSII reaction center. We have generated chloroplast transformants in which the psb30 gene is disrupted and the resulting ?Psb30 cells showed decreased oxygen evolution activity by 15%, grew photosynthetically under moderate light, and displayed increased sensitivity to high light relative to wild type. We conclude that Psb30 is a PSII reaction center subunit and is required for optimal PSII function under high light environments.lld:pubmed
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pubmed-article:21356599pubmed:statusMEDLINElld:pubmed
pubmed-article:21356599pubmed:issn1873-2682lld:pubmed
pubmed-article:21356599pubmed:authorpubmed-author:KashinoYasuhi...lld:pubmed
pubmed-article:21356599pubmed:authorpubmed-author:TakahashiYuic...lld:pubmed
pubmed-article:21356599pubmed:authorpubmed-author:Inoue-Kashino...lld:pubmed
pubmed-article:21356599pubmed:copyrightInfoCopyright © 2011 Elsevier B.V. All rights reserved.lld:pubmed
pubmed-article:21356599pubmed:issnTypeElectroniclld:pubmed
pubmed-article:21356599pubmed:volume104lld:pubmed
pubmed-article:21356599pubmed:ownerNLMlld:pubmed
pubmed-article:21356599pubmed:authorsCompleteYlld:pubmed
pubmed-article:21356599pubmed:pagination220-8lld:pubmed
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pubmed-article:21356599pubmed:articleTitlePsb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii.lld:pubmed
pubmed-article:21356599pubmed:affiliationGraduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.lld:pubmed
pubmed-article:21356599pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21356599pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed