| pubmed-article:21295278 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0018787 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0872076 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0050668 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0279266 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C2698650 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:21295278 | lifeskim:mentions | umls-concept:C0443268 | lld:lifeskim |
| pubmed-article:21295278 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:21295278 | pubmed:dateCreated | 2011-2-7 | lld:pubmed |
| pubmed-article:21295278 | pubmed:abstractText | Efficient differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to a variety of lineages requires step-wise approaches replicating the key commitment stages found during embryonic development. Here we show that expression of PdgfR-? segregates mouse ESC-derived Flk-1 mesoderm into Flk-1(+)PdgfR-?(+) cardiac and Flk-1(+)PdgfR-?(-) hematopoietic subpopulations. By monitoring Flk-1 and PdgfR-? expression, we found that specification of cardiac mesoderm and cardiomyocytes is determined by remarkably small changes in levels of Activin/Nodal and BMP signaling. Translation to human ESCs and iPSCs revealed that the emergence of cardiac mesoderm could also be monitored by coexpression of KDR and PDGFR-? and that this process was similarly dependent on optimal levels of Activin/Nodal and BMP signaling. Importantly, we found that individual mouse and human pluripotent stem cell lines require optimization of these signaling pathways for efficient cardiac differentiation, illustrating a principle that may well apply in other contexts. | lld:pubmed |
| pubmed-article:21295278 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21295278 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21295278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21295278 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21295278 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:21295278 | pubmed:issn | 1875-9777 | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:KellerGordonG | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:EllisJamesJ | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:GagliardiMark... | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:KattmanSteven... | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:HottaAkitsuA | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:DuboisNicole... | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:NiapourMaryam... | lld:pubmed |
| pubmed-article:21295278 | pubmed:author | pubmed-author:WittyAlec DAD | lld:pubmed |
| pubmed-article:21295278 | pubmed:copyrightInfo | Copyright © 2011 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:21295278 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21295278 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:21295278 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:21295278 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21295278 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21295278 | pubmed:pagination | 228-40 | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:meshHeading | pubmed-meshheading:21295278... | lld:pubmed |
| pubmed-article:21295278 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21295278 | pubmed:articleTitle | Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. | lld:pubmed |
| pubmed-article:21295278 | pubmed:affiliation | McEwen Center for Regenerative Medicine, University Health Network, Toronto, Ontario, Canada. | lld:pubmed |
| pubmed-article:21295278 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21295278 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21295278 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:21295278 | lld:pubmed |
