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21259065

Source:http://linkedlifedata.com/resource/pubmed/id/21259065

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pubmed-article:21259065pubmed:abstractTextWild watermelon (Citrullus lanatus) is a xerophyte native to the Kalahari Desert, Africa. To better understand the molecular mechanisms of drought resistance in this plant, we examined changes in the proteome in response to water deficit. Wild watermelon leaves showed decreased transpiration and a concomitant increase in leaf temperature under water deficit conditions. Comparison of the proteome of stressed plants with that of unstressed plants by two-dimensional gel electrophoresis revealed that the intensity of 40 spots increased in response to the stress, and the intensity of 11 spots decreased. We positively identified 23 stress-induced and 6 stress-repressed proteins by mass spectrometry and database analyses. Interestingly, 15 out of the 23 up-regulated proteins (65% of annotated up-regulated proteins) were heat shock proteins (HSPs). Especially, 10 out of the 15 up-regulated HSPs belonged to the small heat shock protein (sHSP) family. Other stress-induced proteins included those related to antioxidative defense and carbohydrate metabolism. Fifteen distinct cDNA sequences encoding the sHSP were characterized from wild watermelon. Quantitative real-time PCR analysis of the representative sHSP genes revealed strong transcriptional up-regulation in the leaves under water deficit. Moreover, immunoblot analysis confirmed that protein abundance of sHSPs was massively increased under water deficit. Overall, these observations suggest that the defense response of wild watermelon may involve orchestrated regulation of a diverse array of functional proteins related to cellular defense and metabolism, of which HSPs may play a pivotal role on the protection of the plant under water deficit in the presence of strong light.lld:pubmed
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pubmed-article:21259065pubmed:authorpubmed-author:YoshidaKazuoKlld:pubmed
pubmed-article:21259065pubmed:authorpubmed-author:YoshimuraKazu...lld:pubmed
pubmed-article:21259065pubmed:authorpubmed-author:YokotaAkihoAlld:pubmed
pubmed-article:21259065pubmed:authorpubmed-author:AkashiKinyaKlld:pubmed
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pubmed-article:21259065pubmed:authorpubmed-author:KajikawaMasat...lld:pubmed
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pubmed-article:21259065pubmed:year2011lld:pubmed
pubmed-article:21259065pubmed:articleTitleDynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit.lld:pubmed
pubmed-article:21259065pubmed:affiliationGraduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan. akashi@bs.naist.jplld:pubmed
pubmed-article:21259065pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21259065pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed