| pubmed-article:21253968 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21253968 | lifeskim:mentions | umls-concept:C0005889 | lld:lifeskim |
| pubmed-article:21253968 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:21253968 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:21253968 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:21253968 | lifeskim:mentions | umls-concept:C0298163 | lld:lifeskim |
| pubmed-article:21253968 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:21253968 | pubmed:dateCreated | 2011-1-21 | lld:pubmed |
| pubmed-article:21253968 | pubmed:abstractText | We describe here the development of a chemifluorescent competitive enzyme-linked immunosorbent assay (ELISA) that quantifies marinobufagenin (MBG) levels in biological fluids. Based on a polyclonal antibody raised against a novel MBG-bovine serum albumin conjugate, this assay achieved an MBG detection limit of less than 9 pg/mL. MBG levels in various rat urine and serum samples were effectively determined using this methodology. Interassay variability averaged 9.8%, while intra-assay variability averaged 1.9 and 2.5% in representative serum and urine samples, respectively. Recovery of exogenously added MBG averaged 106%, and parallelism data further established the accuracy of the assay. Employment of this assay to detect MBG abnormalities represents a powerful tool for the possible diagnosis, prevention and management of human hypertensive states, particularly preeclampsia. | lld:pubmed |
| pubmed-article:21253968 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21253968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21253968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21253968 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21253968 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:21253968 | pubmed:issn | 1532-4230 | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:LiJingJ | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:RomoDanielD | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:PettitGeorge... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:KamanoYoshiak... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:NogawaToshihi... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:PuschettJules... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:BerghmanLuc... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:XuJun-PingJP | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:Abi-GhanemDaa... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:UddinMohammad... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:HorvatDarijan... | lld:pubmed |
| pubmed-article:21253968 | pubmed:author | pubmed-author:LaiXinzhongX | lld:pubmed |
| pubmed-article:21253968 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21253968 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:21253968 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21253968 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21253968 | pubmed:pagination | 31-46 | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
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| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:meshHeading | pubmed-meshheading:21253968... | lld:pubmed |
| pubmed-article:21253968 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21253968 | pubmed:articleTitle | A chemifluorescent immunoassay for the determination of marinobufagenin in body fluids. | lld:pubmed |
| pubmed-article:21253968 | pubmed:affiliation | Department of Poultry Science, Texas A&M University, College Station, Texas, USA. | lld:pubmed |
| pubmed-article:21253968 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21253968 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:21253968 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21253968 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
