| pubmed-article:2123854 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0004594 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0059100 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:2123854 | lifeskim:mentions | umls-concept:C0059935 | lld:lifeskim |
| pubmed-article:2123854 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:2123854 | pubmed:dateCreated | 1991-1-24 | lld:pubmed |
| pubmed-article:2123854 | pubmed:abstractText | We isolated a thermophilic bacterium that produces both xylanase and beta-xylosidase. Based on taxonomical research, this bacterium was identified as Bacillus stearothermophilus. Each extracellular enzyme was separated by hydrophobic chromatography by using a Toyopearl HW-65 column, followed by gel filtration with a Sephacryl S-200 column. Each enzyme in the culture was further purified to homogeneity (62-fold for xylanase and 72-fold for beta-xylosidase) by using a fast protein liquid chromatography system with a Mono Q HR 5/5 column. The optimum temperatures were 60 degrees C for xylanase and 70 degrees C for beta-xylosidase. The isoelectric points and molecular masses were 5.1 and 39.5 kDa for xylanase and 4.2 and 150 kDa for beta-xylosidase, respectively. Heat treatment at 60 degrees C for 1 h did not cause inhibition of the activities of these enzymes. The action of the two enzymes on xylan gave only xylose. | lld:pubmed |
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| pubmed-article:2123854 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2123854 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2123854 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2123854 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2123854 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2123854 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2123854 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2123854 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2123854 | pubmed:author | pubmed-author:WatanabeTT | lld:pubmed |
| pubmed-article:2123854 | pubmed:author | pubmed-author:KawamuraYY | lld:pubmed |
| pubmed-article:2123854 | pubmed:author | pubmed-author:ShinkeRR | lld:pubmed |
| pubmed-article:2123854 | pubmed:author | pubmed-author:KohnoAA | lld:pubmed |
| pubmed-article:2123854 | pubmed:author | pubmed-author:NanmoriTT | lld:pubmed |
| pubmed-article:2123854 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2123854 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:2123854 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2123854 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2123854 | pubmed:pagination | 6669-72 | lld:pubmed |
| pubmed-article:2123854 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:meshHeading | pubmed-meshheading:2123854-... | lld:pubmed |
| pubmed-article:2123854 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2123854 | pubmed:articleTitle | Purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated Bacillus stearothermophilus strain. | lld:pubmed |
| pubmed-article:2123854 | pubmed:affiliation | Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University, Japan. | lld:pubmed |
| pubmed-article:2123854 | pubmed:publicationType | Journal Article | lld:pubmed |
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