Linked Life Data

2123400

Source:http://linkedlifedata.com/resource/pubmed/id/2123400

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:2123400rdf:typepubmed:Citationlld:pubmed
pubmed-article:2123400lifeskim:mentionsumls-concept:C0220806lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C0085080lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C0017337lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C0003591lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C1521970lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C1704241lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C0205485lld:lifeskim
pubmed-article:2123400lifeskim:mentionsumls-concept:C1677784lld:lifeskim
pubmed-article:2123400pubmed:issue1lld:pubmed
pubmed-article:2123400pubmed:dateCreated1991-1-15lld:pubmed
pubmed-article:2123400pubmed:abstractTextChinese hamster ovary cells transfected with the human apolipoprotein A-I gene linked to the human metallothionein gene promoter region secrete large quantities of apolipoprotein A-I (7.1 +/- 0.4% total secreted protein) in the presence of zinc. Approx. 16% of the secreted apolipoprotein A-I is complexed with lipid and can be isolated ultracentrifugally at d less than or equal to 1.21 g/ml. The latter complexes are composed of discs and vesicles as judged by electron microscopy and can be further separated by column chromatography into three fractions: fraction I, mostly vesicles (60-260 nm) and large discs (18-20 nm diameter); fraction II, discs 14.2 +/- 2.6 nm diameter; and fraction III, nonresolvable by electron microscopy. The latter fraction is extremely lipid-poor (94% protein, 6% phospholipid); in contrast, the protein, phospholipid and unesterified cholesterol content for the other fractions are 43, 33 and 24%, respectively, for fraction I and 53, 33 and 14%, respectively, for fraction II. Fraction II particles contain three and four apolipoprotein A-Is per particle as determined by protein crosslinking while large structures in fraction I contain primarily six to seven apolipoprotein A-Is per particle. Following incubation with purified lecithin: cholesterol acyltransferase, discoidal particles were transformed into apparent spherical particles 12.9 +/- 3.4 nm diameter; this transformation coincided with 19-21% conversion of unesterified cholesterol to esterified cholesterol. The apolipoprotein A-I-lipid complexes isolated from Chinese hamster ovary cell media are similar to nascent HDL found in plasma of lecithin:cholesterol acyltransferase-deficient patients and those secreted by the human hepatoma line, Hep G2. The ability of the Chinese hamster ovary cell nascent HDL-like particles to undergo transformation in the presence of purified lecithin:cholesterol acyltransferase indicates that they are functional particles.lld:pubmed
pubmed-article:2123400pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:languageenglld:pubmed
pubmed-article:2123400pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:citationSubsetIMlld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:2123400pubmed:statusMEDLINElld:pubmed
pubmed-article:2123400pubmed:monthOctlld:pubmed
pubmed-article:2123400pubmed:issn0006-3002lld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:VigneJ LJLlld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:ForteT MTMlld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:NordhausenR...lld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:McCallM RMRlld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:MalloryJ BJBlld:pubmed
pubmed-article:2123400pubmed:authorpubmed-author:AmacherSSlld:pubmed
pubmed-article:2123400pubmed:issnTypePrintlld:pubmed
pubmed-article:2123400pubmed:day22lld:pubmed
pubmed-article:2123400pubmed:volume1047lld:pubmed
pubmed-article:2123400pubmed:ownerNLMlld:pubmed
pubmed-article:2123400pubmed:authorsCompleteYlld:pubmed
pubmed-article:2123400pubmed:pagination11-8lld:pubmed
pubmed-article:2123400pubmed:dateRevised2008-11-21lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:meshHeadingpubmed-meshheading:2123400-...lld:pubmed
pubmed-article:2123400pubmed:year1990lld:pubmed
pubmed-article:2123400pubmed:articleTitlePhysical and chemical characteristics of apolipoprotein A-I-lipid complexes produced by Chinese hamster ovary cells transfected with the human apolipoprotein A-I gene.lld:pubmed
pubmed-article:2123400pubmed:affiliationLawrence Berkeley Laboratory, University of California 94702.lld:pubmed
pubmed-article:2123400pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2123400pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:2123400pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2123400lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:2123400lld:pubmed