| pubmed-article:2120190 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C1429528 | lld:lifeskim |
| pubmed-article:2120190 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2120190 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2120190 | pubmed:dateCreated | 1990-11-14 | lld:pubmed |
| pubmed-article:2120190 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2120190 | pubmed:abstractText | The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of proteins encoded by the cloned fragment in a T7 expression system revealed a 32,000-dalton protein band, which correlated with the ability of cells to transport citrate. Energy-dependent [1,5-14C]citrate transport was found with membrane vesicles prepared from E. coli cells harboring the citrate permease-expressing plasmid. The gene encoding citrate transport activity, citP, was located on the cloned fragment by introducing a site-specific mutation that abolished citrate transport and resulted in a truncated form of the 32,000-dalton expression product. The nucleotide sequence for a 2.2-kilobase fragment that includes the citP gene contained an open reading frame of 1,325 base pairs coding for a very hydrophobic protein of 442 amino acids, which shows no sequence homology with known citrate carriers. | lld:pubmed |
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| pubmed-article:2120190 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2120190 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2120190 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2120190 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2120190 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2120190 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2120190 | pubmed:author | pubmed-author:DavidSS | lld:pubmed |
| pubmed-article:2120190 | pubmed:author | pubmed-author:SimonsGG | lld:pubmed |
| pubmed-article:2120190 | pubmed:author | pubmed-author:de VosW MWM | lld:pubmed |
| pubmed-article:2120190 | pubmed:author | pubmed-author:DriessenA JAJ | lld:pubmed |
| pubmed-article:2120190 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:2120190 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2120190 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:2120190 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2120190 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2120190 | pubmed:pagination | 5789-94 | lld:pubmed |
| pubmed-article:2120190 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
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| pubmed-article:2120190 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2120190 | pubmed:articleTitle | Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene. | lld:pubmed |
| pubmed-article:2120190 | pubmed:affiliation | Molecular Genetics Group, Netherlands Institute for Dairy Research, Ede. | lld:pubmed |
| pubmed-article:2120190 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2120190 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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