| pubmed-article:21187066 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C1333336 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C0215848 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C2348977 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C1822686 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C2348110 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:21187066 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:21187066 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21187066 | pubmed:dateCreated | 2011-1-31 | lld:pubmed |
| pubmed-article:21187066 | pubmed:abstractText | Hypoxia has emerged as a key determinant of osteogenesis. HIF-1? is the transcription factor mediating hypoxia responses that include induction of VEGF and related bone induction. Inflammatory signals antagonize bone repair via the NF-?B pathway. The present investigation explored the functional relationship of hypoxia (HIF-1? function) and inflammatory signaling (NF-?B) in stem like and osteoprogenitor cell lines. The potential interaction between HIF-1? and NF-?B signaling was explored by co-transfection studies in hFOB with p65, HIF-1? and 9x-HRE-luc or HIF-1? target genes reporter plasmids. Nuclear cross-talk was directly tested using the mammalian Gal4/VP16 two-hybrid, and confirmed by co-immunoprecipitation/western blotting assays. The results show that inflammatory stimulation (TNF-? treatment) causes a marked inhibition of HIF-1? function at the HRE in all cell lines studied. Also, co-transfection with p65 expression vector leads to reduced hVEGFp transcription after DFO-induced hypoxia. However, TNF-? treatment had little effect on HIF-1? mRNA levels. The functional interaction of Gal4-HIF-1? and VP16-p300 fusion proteins is effectively blocked by expression of p65 in a dose dependent manner. It was concluded that NF-?B-mediated inflammatory signaling is able to block HIF-1? transactivation at HRE-encoding genes by direct competition for p300 binding at the promoter. Inflammation may influence the stem cell niche and tissue regeneration by influencing cellular responses to hypoxia. | lld:pubmed |
| pubmed-article:21187066 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21187066 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21187066 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21187066 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:21187066 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:21187066 | pubmed:author | pubmed-author:CooperLyndon... | lld:pubmed |
| pubmed-article:21187066 | pubmed:author | pubmed-author:MendonçaGusta... | lld:pubmed |
| pubmed-article:21187066 | pubmed:author | pubmed-author:AragãoFrancis... | lld:pubmed |
| pubmed-article:21187066 | pubmed:author | pubmed-author:MendonçaDanie... | lld:pubmed |
| pubmed-article:21187066 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:21187066 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21187066 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:21187066 | pubmed:volume | 404 | lld:pubmed |
| pubmed-article:21187066 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21187066 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21187066 | pubmed:pagination | 997-1003 | lld:pubmed |
| pubmed-article:21187066 | pubmed:dateRevised | 2011-10-27 | lld:pubmed |
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| pubmed-article:21187066 | pubmed:meshHeading | pubmed-meshheading:21187066... | lld:pubmed |
| pubmed-article:21187066 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21187066 | pubmed:articleTitle | NF-?B suppresses HIF-1? response by competing for P300 binding. | lld:pubmed |
| pubmed-article:21187066 | pubmed:affiliation | Universidade Católica de Brasília, Pós-Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasília, DF, Brazil. | lld:pubmed |
| pubmed-article:21187066 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21187066 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21187066 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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