| pubmed-article:2118365 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0085979 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0001214 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0600485 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0072014 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:2118365 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:2118365 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2118365 | pubmed:dateCreated | 1990-10-9 | lld:pubmed |
| pubmed-article:2118365 | pubmed:abstractText | The kinetics of proacrosin activation and release from guinea pig spermatozoa during the nonsynchronous acrosome reaction were studied. Epididymal spermatozoa were incubated at 37 degrees C in a defined medium (pH 7.8) containing 1.7 mM Ca2+. After 195 min, 78% of the motile spermatozoa had undergone the acrosome reaction as determined by light microscopy. Acrosin and proacrosin levels in the spermatozoa and medium were measured at the beginning of the incubation period. Most of the total acrosin activity (78%) was associated with the spermatozoa, of which greater than 90% was in the form of proacrosin. Proacrosin represented a small, stable fraction (23%) of the total acrosin in the medium; it did not activate to acrosin while in the medium. After 195 min, a decrease in sperm-associated total acrosin (42%; p less than 0.05) was accompanied by an increase in the total acrosin level in the medium (115%; P less than 0.05). No change in the relative proacrosin content (percent of total acrosin) was evident in either medium or spermatozoa. Additional experiments quantified acrosin and proacrosin during the progression of the acrosome reaction. Both the loss of sperm-associated total acrosin and the increase in total acrosin levels in the medium were highly correlated with the fraction of acrosome-reacted spermatozoa (r = 0.954 and 0.922, respectively; P less than 0.001). However, the rate of acrosin appearance in the medium was only 60% (P less than 0.001) of the rate of acrosin loss from the spermatozoa. The fractional proacrosin content of spermatozoa (94%) and medium (31%) remained unchanged during the acrosome reaction (r = 0.15 and 0.30, respectively; P greater than 0.1).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2118365 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2118365 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2118365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2118365 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2118365 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2118365 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:2118365 | pubmed:author | pubmed-author:ZaneveldL JLJ | lld:pubmed |
| pubmed-article:2118365 | pubmed:author | pubmed-author:AndersonR... | lld:pubmed |
| pubmed-article:2118365 | pubmed:author | pubmed-author:NuzzoN ANA | lld:pubmed |
| pubmed-article:2118365 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2118365 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:2118365 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2118365 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2118365 | pubmed:pagination | 52-60 | lld:pubmed |
| pubmed-article:2118365 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:meshHeading | pubmed-meshheading:2118365-... | lld:pubmed |
| pubmed-article:2118365 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2118365 | pubmed:articleTitle | Proacrosin activation and acrosin release during the guinea pig acrosome reaction. | lld:pubmed |
| pubmed-article:2118365 | pubmed:affiliation | Department of Obstetrics and Gynecology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612. | lld:pubmed |
| pubmed-article:2118365 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2118365 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
