| pubmed-article:21127908 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21127908 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:21127908 | lifeskim:mentions | umls-concept:C0015852 | lld:lifeskim |
| pubmed-article:21127908 | lifeskim:mentions | umls-concept:C0475208 | lld:lifeskim |
| pubmed-article:21127908 | lifeskim:mentions | umls-concept:C0033269 | lld:lifeskim |
| pubmed-article:21127908 | lifeskim:mentions | umls-concept:C0549178 | lld:lifeskim |
| pubmed-article:21127908 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21127908 | pubmed:dateCreated | 2011-4-12 | lld:pubmed |
| pubmed-article:21127908 | pubmed:abstractText | We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reactors (CSTRs) with either hollow fiber cell recycling or cell immobilization for high cell-density culture. In each CSTR substrate supply and product removal are possible. Penicillin production is severely limited by glucose metabolite repression that requires multi-CSTR glucose feeding. An 8-stage C-HCDC lactic acid fermentation resulted in 212.9 g/L of titer and 10.6 g/L/h of productivity, corresponding to 101 and 429% of the comparable lactic acid fed-batch, respectively. The penicillin production model predicted 149% (0.085 g/L/h) of productivity in 8-stage C-HCDC with 40 g/L of cell density and 289% of productivity (0.165 g/L/h) in 7-stage C-HCDC with 60 g/L of cell density compared with referring batch cultivations. A 2-stage C-HCDC ethanol experimental run showed 107% titer and 257% productivity of the batch system having 88.8 g/L of titer and 3.7 g/L/h of productivity. MSC-HCDC can give much higher productivity than batch/fed-batch system, and yield a several percentage higher titer as well. The productivity ratio of MSC-HCDC over batch/fed-batch system is given as a multiplication of system dilution rate of MSC-HCDC and cycle time of batch/fed-batch system. We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody. | lld:pubmed |
| pubmed-article:21127908 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21127908 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21127908 | pubmed:month | May | lld:pubmed |
| pubmed-article:21127908 | pubmed:issn | 1615-7605 | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:ChangHo NamHN | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:KimJungbaeJ | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:LeeSang YupSY | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:KimByoung... | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:FeiQiangQ | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:ChoiJin-dal-r... | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:KimNag-JongNJ | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:JeongChang... | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:KangJongwonJ | lld:pubmed |
| pubmed-article:21127908 | pubmed:author | pubmed-author:KwonSunhoonS | lld:pubmed |
| pubmed-article:21127908 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21127908 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:21127908 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21127908 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21127908 | pubmed:pagination | 419-31 | lld:pubmed |
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| pubmed-article:21127908 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21127908 | pubmed:articleTitle | Multi-stage high cell continuous fermentation for high productivity and titer. | lld:pubmed |
| pubmed-article:21127908 | pubmed:affiliation | Department of Chemical and Biomolecular Engineering, KAIST (Korea Advanced Institute of Science and Technology), Daejeon, Korea. hnchang@kaist.edu | lld:pubmed |
| pubmed-article:21127908 | pubmed:publicationType | Journal Article | lld:pubmed |
