| pubmed-article:2111184 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2111184 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:2111184 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:2111184 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:2111184 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:2111184 | lifeskim:mentions | umls-concept:C0439536 | lld:lifeskim |
| pubmed-article:2111184 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2111184 | pubmed:dateCreated | 1990-6-26 | lld:pubmed |
| pubmed-article:2111184 | pubmed:abstractText | One-cell embryos from several different strains of mice have been cultured to the blastocyst stage in CZB medium. CZB medium can be used to culture CF1 x B6SJLF1/J 1-cell embryos to the blastocyst stage provided glucose is introduced into the medium on Day 3 of culture. The amount of glucose required for embryo development was titrated using a concentration range of 5.5 to 49.5 mM. With the exception of the highest concentration, all glucose levels tested supported 65-85% development to the morula and blastocyst stages. Variations of CZB medium were tested for their ability to support the development of 1-cell embryos from 4 strains of mice. For embryos from CF1 and DBA/2J (both x B6SJLF1/J) mice, which exhibit a "2-cell block" to development in vitro, CZB medium containing glutamine with the addition of glucose on Day 3 supported optimum development from the 1-cell stage to morula and blastocysts (79% and 87%). For embryos from B6D2F1/J and CD1 female mice (both x B6SJLF1/J males), which do not exhibit a "2-cell block" to in vitro development, optimum development to morula and blastocyst stages (95% and 50%) was in CZB medium containing both glutamine and glucose from the start of culture. | lld:pubmed |
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| pubmed-article:2111184 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2111184 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2111184 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2111184 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2111184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2111184 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2111184 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2111184 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2111184 | pubmed:issn | 0006-3363 | lld:pubmed |
| pubmed-article:2111184 | pubmed:author | pubmed-author:LewisJ LJL | lld:pubmed |
| pubmed-article:2111184 | pubmed:author | pubmed-author:ZiomekC ACA | lld:pubmed |
| pubmed-article:2111184 | pubmed:author | pubmed-author:TorresII | lld:pubmed |
| pubmed-article:2111184 | pubmed:author | pubmed-author:ChatotC LCL | lld:pubmed |
| pubmed-article:2111184 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2111184 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:2111184 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2111184 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2111184 | pubmed:pagination | 432-40 | lld:pubmed |
| pubmed-article:2111184 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:2111184 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2111184 | pubmed:articleTitle | Development of 1-cell embryos from different strains of mice in CZB medium. | lld:pubmed |
| pubmed-article:2111184 | pubmed:affiliation | Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545. | lld:pubmed |
| pubmed-article:2111184 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2111184 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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