| pubmed-article:21106538 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21106538 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:21106538 | lifeskim:mentions | umls-concept:C0538674 | lld:lifeskim |
| pubmed-article:21106538 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:21106538 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:21106538 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:21106538 | pubmed:dateCreated | 2011-1-31 | lld:pubmed |
| pubmed-article:21106538 | pubmed:abstractText | Heme oxygenase-1 (HO-1) enzyme plays a critical role in metabolizing the excess heme generated during hemolysis. Our previous studies suggested that during intravascular hemolysis the expression of HO-1 protein is not sufficient to reduce the oxidative burden of free heme in the vasculature. This led us to hypothesize that a post-translational mechanism of control exists for HO-1 expression. Micro-RNAs (miRNA) affect gene expression by post-transcriptional gene regulation of transcripts. We performed in silico analysis for the human HMOX1-3' untranslated region (3' UTR) and identified candidate miRNA binding sites. Two candidate miRNAs, miR-377 and miR-217, were cloned and co-transfected with a luciferase vector containing the human HMOX1-3'UTR region. The combination of miR-377 and miR-217 produced a 58% reduction in HMOX1-3'UTR luciferase reporter expression compared with controls. The same constructs were then used to assess how overexpression of miR-217 and miR-377 affected HO-1 levels after induction with hemin. Cells transfected with the combination of miR-377 and miR-217 exhibited no change in HMOX1 mRNA levels, but a significant reduction in HMOX1 (HO-1) protein expression and enzyme activity compared with non-transfected hemin-stimulated controls. Transfection with either miR-377 or miR-217 alone did not produce a significant decrease in HO-1 protein expression or enzyme activity. Knockdown of miR-217 and miR-377 in combination leads to up-regulation of HO-1 protein. Exposure to hemin induced a significant reduction in miR-217 expression and a trend toward decreased miR-377 expression in two different cells lines. In summary, these data suggests that the combination of miR-377 and miR-217 help regulate HO-1 protein expression in the presence of hemin. | lld:pubmed |
| pubmed-article:21106538 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21106538 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21106538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21106538 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21106538 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:21106538 | pubmed:issn | 1083-351X | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:SteerClifford... | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:VercellottiGr... | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:SubramanianSu... | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:NguyenJuliaJ | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:ThayanithyVen... | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:BeckmanJoan... | lld:pubmed |
| pubmed-article:21106538 | pubmed:author | pubmed-author:ChenChunsengC | lld:pubmed |
| pubmed-article:21106538 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21106538 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:21106538 | pubmed:volume | 286 | lld:pubmed |
| pubmed-article:21106538 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21106538 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21106538 | pubmed:pagination | 3194-202 | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:meshHeading | pubmed-meshheading:21106538... | lld:pubmed |
| pubmed-article:21106538 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21106538 | pubmed:articleTitle | Regulation of heme oxygenase-1 protein expression by miR-377 in combination with miR-217. | lld:pubmed |
| pubmed-article:21106538 | pubmed:affiliation | Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA. | lld:pubmed |
| pubmed-article:21106538 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21106538 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21106538 | lld:entrezgene |
