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| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C0028580 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C0035124 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C1553879 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C1549542 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C1511545 | lld:lifeskim |
| pubmed-article:2110515 | lifeskim:mentions | umls-concept:C0048878 | lld:lifeskim |
| pubmed-article:2110515 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2110515 | pubmed:dateCreated | 1990-6-11 | lld:pubmed |
| pubmed-article:2110515 | pubmed:abstractText | Calcium has been implicated as a mediator of cell injury in ischemia and reperfusion, but direct measurements of Ca2+ are required to refine this idea. We used nuclear magnetic resonance spectroscopy and the Ca2+ indicator 5F-BAPTA to measure [Ca2+]i in perfused ferret hearts. Several lines of evidence are presented to show that loading with the acetoxymethyl ester of 5F-BAPTA is not significantly complicated by accumulation of partially de-esterified metabolites, compartmentalization into mitochondria, or disproportionate uptake into endothelial cells. During 20 minutes of total global ischemia at 30 degrees C, time-averaged [Ca2+]i increased significantly, reaching peak values roughly three times control at 15-20 minutes. Reperfusion resulted in a persistent elevation of [Ca2+]i during the first 5 minutes, but not afterward. Although the nonlinear response of 5F-BAPTA to [Ca2+] leads to underestimation of the true time-averaged [Ca2+]i, the measured alterations of intracellular Ca2+ homeostasis during ischemia are large compared with the likely errors in quantification. Phosphorus nuclear magnetic resonance spectroscopy of 5F-BAPTA-loaded hearts reveals changes during ischemia similar to those recorded previously in hearts not containing a Ca2+ indicator. Developed pressure recovers to only 50% of control values during reflow, indicating that the presence of 5F-BAPTA in the cytosol does not protect against stunning, at least when the extracellular calcium concentration has been raised to 8 mM. We conclude that 5F-BAPTA provides useful measurements that reveal that time-averaged [Ca2+]i rises during ischemia and returns to control levels soon after reperfusion. | lld:pubmed |
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| pubmed-article:2110515 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2110515 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2110515 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2110515 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2110515 | pubmed:month | May | lld:pubmed |
| pubmed-article:2110515 | pubmed:issn | 0009-7330 | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:MarbanEE | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:GutV AVA | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:KitakazeMM | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:ChackoV PVP | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:KoretsuneYY | lld:pubmed |
| pubmed-article:2110515 | pubmed:author | pubmed-author:PikeM MMM | lld:pubmed |
| pubmed-article:2110515 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2110515 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:2110515 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2110515 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2110515 | pubmed:pagination | 1255-67 | lld:pubmed |
| pubmed-article:2110515 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:2110515 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2110515 | pubmed:articleTitle | Quantification of [Ca2+]i in perfused hearts. Critical evaluation of the 5F-BAPTA and nuclear magnetic resonance method as applied to the study of ischemia and reperfusion. | lld:pubmed |
| pubmed-article:2110515 | pubmed:affiliation | Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland. | lld:pubmed |
| pubmed-article:2110515 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2110515 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2110515 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2110515 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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