| pubmed-article:21098666 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0010240 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0040715 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C1522702 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0599718 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0599813 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C0599893 | lld:lifeskim |
| pubmed-article:21098666 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:21098666 | pubmed:issue | 50 | lld:pubmed |
| pubmed-article:21098666 | pubmed:dateCreated | 2011-3-22 | lld:pubmed |
| pubmed-article:21098666 | pubmed:abstractText | Coxiella burnetii is an obligate intracellular bacterial pathogen responsible for acute and chronic Q fever. This bacterium harbors a type IV secretion system (T4SS) highly similar to the Dot/Icm of Legionella pneumophila that is believed to be essential for its infectivity. Protein substrates of the Coxiella T4SS are predicted to facilitate the biogenesis of a phagosome permissive for its intracellular growth. However, due to the lack of genetic systems, protein transfer by the C. burnetii Dot/Icm has not been demonstrated. In this study, we report the identification of 32 substrates of the C. burnetii Dot/Icm system using a fluorescence-based ?-lactamase (TEM1) translocation assay as well as the calmodulin-dependent adenylate cyclase (CyaA) assay in the surrogate host L. pneumophila. Notably, 26 identified T4SS substrates are hypothetical proteins without predicted function. Candidate secretion substrates were obtained by using (i) a genetic screen to identify C. burnetii proteins interacting with DotF, a component of the T4SS, and (ii) bioinformatic approaches to retrieve candidate genes that harbor characteristics associated with previously reported substrates of the Dot/Icm system from both C. burnetii and L. pneumophila. Moreover, we have developed a shuttle plasmid that allows the expression of recombinant proteins in C. burnetii as TEM fusion products. Using this system, we demonstrated that a Dot/Icm substrate identified with L. pneumophila was also translocated by C. burnetii in a process that requires its C terminus, providing direct genetic evidence of a functional T4SS in C. burnetii. | lld:pubmed |
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| pubmed-article:21098666 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21098666 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21098666 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21098666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21098666 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21098666 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:21098666 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:XXX | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:SamuelJames... | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:LuoZhao-QingZ... | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:BangaSimranS | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:MertensKatjaK | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:WeberMary MMM | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:GorbaslievaIv... | lld:pubmed |
| pubmed-article:21098666 | pubmed:author | pubmed-author:TanYunhaoY | lld:pubmed |
| pubmed-article:21098666 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21098666 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:21098666 | pubmed:volume | 107 | lld:pubmed |
| pubmed-article:21098666 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21098666 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21098666 | pubmed:pagination | 21755-60 | lld:pubmed |
| pubmed-article:21098666 | pubmed:dateRevised | 2011-8-1 | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:meshHeading | pubmed-meshheading:21098666... | lld:pubmed |
| pubmed-article:21098666 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:21098666 | pubmed:articleTitle | Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii. | lld:pubmed |
| pubmed-article:21098666 | pubmed:affiliation | Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science Center, College Station, TX 77843, USA. | lld:pubmed |
| pubmed-article:21098666 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21098666 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
