| pubmed-article:21092188 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21092188 | lifeskim:mentions | umls-concept:C0086661 | lld:lifeskim |
| pubmed-article:21092188 | lifeskim:mentions | umls-concept:C0007131 | lld:lifeskim |
| pubmed-article:21092188 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:21092188 | lifeskim:mentions | umls-concept:C0596290 | lld:lifeskim |
| pubmed-article:21092188 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:21092188 | pubmed:dateCreated | 2010-12-14 | lld:pubmed |
| pubmed-article:21092188 | pubmed:abstractText | MicroRNAs are important gene regulators that potentially play a profound role in tumorigenesis. Increasing evidence indicates that miR-145 is a tumor suppressor capable of inhibiting breast and colon cancer cell growth both in vitro and in vivo. However, the biological function of miR-145 in non-small cell lung cancer (NSCLC) is largely unknown. In colon cancer cells, c-Myc is a confirmed direct target for miR-145. The aim of this work was to investigate the effect of miR-145 and c-Myc on proliferation of NSCLC cells, using the NSCLC cell lines A549 and H23 as models. We determined the expression level of miR-145 in tumor tissues relative to adjacent non-tumor tissues, and in NSCLC cell lines relative to non-malignant lung cells. Downregulation of miR-145 was seen in tumor tissues and the two NSCLC cell lines by real-time quantitative reverse transcription polymerase chain reaction. MTT and focus formation assays were conducted to measure cell proliferation rates. Cell growth was inhibited and the G1/S transition was blocked by miR-145 in transfection assays of A549 and H23 cells. We further showed that c-Myc was a direct target for miR-145. Introduction of miR-145 dramatically suppressed the c-Myc/eIF4E pathway, which was demonstrated to be crucial for cell proliferation in NSCLC cells. Furthermore, we found that CDK4 was regulated by miR-145 in cell cycle control. Taken together, our study results demonstrate that miR-145 inhibits proliferation of NSCLC cells through c-Myc. Increasing miR-145 expression may provide a novel approach for the treatment of NSCLC. | lld:pubmed |
| pubmed-article:21092188 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21092188 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21092188 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21092188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21092188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21092188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21092188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21092188 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21092188 | pubmed:issn | 1756-9966 | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:HsuR YRY | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:SIMY BYB | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:GuoYongY | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:ChenZheZ | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:HuJianJ | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:DengAnmeiA | lld:pubmed |
| pubmed-article:21092188 | pubmed:author | pubmed-author:ZengHuazongH | lld:pubmed |
| pubmed-article:21092188 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21092188 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:21092188 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21092188 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21092188 | pubmed:pagination | 151 | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:meshHeading | pubmed-meshheading:21092188... | lld:pubmed |
| pubmed-article:21092188 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:21092188 | pubmed:articleTitle | miRNA-145 inhibits non-small cell lung cancer cell proliferation by targeting c-Myc. | lld:pubmed |
| pubmed-article:21092188 | pubmed:affiliation | National Clinical Research Base of Traditional Chinese Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou 310006, China. | lld:pubmed |
| pubmed-article:21092188 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21092188 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:4609 | entrezgene:pubmed | pubmed-article:21092188 | lld:entrezgene |
| entrez-gene:406937 | entrezgene:pubmed | pubmed-article:21092188 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21092188 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:21092188 | lld:entrezgene |
