| pubmed-article:21088885 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21088885 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:21088885 | lifeskim:mentions | umls-concept:C0886515 | lld:lifeskim |
| pubmed-article:21088885 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:21088885 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:21088885 | pubmed:dateCreated | 2010-12-24 | lld:pubmed |
| pubmed-article:21088885 | pubmed:abstractText | We have investigated the dynamics of the free [Ca(2+)] inside the secretory granules of neurosecretory PC12 and INS1 cells using a low-Ca(2+)-affinity aequorin chimera fused to synaptobrevin-2. The steady-state secretory granule [Ca(2+)] ([Ca(2+)](SG)] was around 20-40 ?M in both cell types, about half the values previously found in chromaffin cells. Inhibition of SERCA-type Ca(2+) pumps with thapsigargin largely blocked Ca(2+) uptake by the granules in Ca(2+)-depleted permeabilized cells, and the same effect was obtained when the perfusion medium lacked ATP. Consistently, the SERCA-type Ca(2+) pump inhibitor benzohydroquinone induced a rapid release of Ca(2+) from the granules both in intact and permeabilized cells, suggesting that the continuous activity of SERCA-type Ca(2+) pumps is essential to maintain the steady-state [Ca(2+)](SG). Both inositol 1,4,5-trisphosphate (InsP(3)) and caffeine produced a rapid Ca(2+) release from the granules, suggesting the presence of InsP(3) and ryanodine receptors in the granules. The response to high-K(+) depolarization was different in both cell types, a decrease in [Ca(2+)](SG) in PC12 cells and an increase in [Ca(2+)](SG) in INS1 cells. The difference may rely on the heterogeneous response of different vesicle populations in each cell type. Finally, increasing the glucose concentration triggered a decrease in [Ca(2+)](SG) in INS1 cells. In conclusion, our data show that the secretory granules of PC12 and INS1 cells take up Ca(2+) through SERCA-type Ca(2+) pumps and can release it through InsP(3) and ryanodine receptors, supporting the hypothesis that secretory granule Ca(2+) may be released during cell stimulation and contribute to secretion. | lld:pubmed |
| pubmed-article:21088885 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21088885 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21088885 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21088885 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21088885 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21088885 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21088885 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21088885 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:21088885 | pubmed:issn | 1573-6830 | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:AlvarezJavier... | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:MonteroMayteM | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:FonterizRosal... | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:MorenoAlfredo... | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:LobatónCarmen... | lld:pubmed |
| pubmed-article:21088885 | pubmed:author | pubmed-author:SantoDomingoJ... | lld:pubmed |
| pubmed-article:21088885 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21088885 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:21088885 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21088885 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21088885 | pubmed:pagination | 1267-74 | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:meshHeading | pubmed-meshheading:21088885... | lld:pubmed |
| pubmed-article:21088885 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:21088885 | pubmed:articleTitle | Ca2+ dynamics in the secretory vesicles of neurosecretory PC12 and INS1 cells. | lld:pubmed |
| pubmed-article:21088885 | pubmed:affiliation | Instituto de Biología y Genética Molecular, Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid and Consejo Superior de Investigaciones Científicas, Ramón y Cajal, 7, 47005 Valladolid, Spain. | lld:pubmed |
| pubmed-article:21088885 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21088885 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
