| pubmed-article:21071079 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C2700384 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0026385 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0032486 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C1704666 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C1517892 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0208973 | lld:lifeskim |
| pubmed-article:21071079 | lifeskim:mentions | umls-concept:C0066201 | lld:lifeskim |
| pubmed-article:21071079 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21071079 | pubmed:dateCreated | 2010-11-26 | lld:pubmed |
| pubmed-article:21071079 | pubmed:abstractText | To improve transfection efficiency and reduce the cytotoxicity of polymeric gene vectors, reducible polycations (RPC) were synthesized from low molecular weight (MW) branched polyethyleneimine (bPEI) via thiolation and oxidation. RPC (RPC-bPEI(0.8 kDa)) possessed MW of 5 kDa-80 kDa, and 50%-70% of the original proton buffering capacity of bPEI(0.8 kDa) was preserved in the final product. The cytotoxicity of RPC-bPEI(0.8 kDa) was 8-19 times less than that of the gold standard of polymeric transfection reagents, bPEI(25 kDa). Although bPEI(0.8 kDa) exhibited poor gene condensing capacities (?2 ?m at a weight ratio (WR) of 40), RPC-bPEI(0.8 kDa) effectively condensed plasmid DNA (pDNA) at a WR of 2. Moreover, RPC-bPEI(0.8 kDa)/pDNA (WR ?2) formed 100-200 nm-sized particles with positively charged surfaces (20-35 mV). In addition, the results of the present study indicated that thiol/polyanions triggered the release of pDNA from RPC-bPEI(0.8 kDa)/pDNA via the fragmentation of RPC-bPEI(0.8 kDa) and ion-exchange. With negligible polyplex-mediated cytotoxicity, the transfection efficiencies of RPC-bPEI(0.8 kDa)/pDNA were approximately 1200-1500-fold greater than that of bPEI(0.8 kDa)/pDNA and were equivalent or superior (?7-fold) to that of bPEI(25 kDa)/pDNA. Interestingly, the distribution of high MW RPC-bPEI(0.8 kDa)/pDNA in the nucleus of the cell was higher than that of low MW RPC-bPEI(0.8 kDa)/pDNA. Thus, the results of the present study suggest that RPC-bPEI(0.8 kDa) has the potential to effectively deliver genetic materials with lower levels of toxicity. | lld:pubmed |
| pubmed-article:21071079 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21071079 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21071079 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21071079 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:21071079 | pubmed:issn | 1878-5905 | lld:pubmed |
| pubmed-article:21071079 | pubmed:author | pubmed-author:BaeYou HanYH | lld:pubmed |
| pubmed-article:21071079 | pubmed:author | pubmed-author:KangHo-JungHJ | lld:pubmed |
| pubmed-article:21071079 | pubmed:author | pubmed-author:KangHan... | lld:pubmed |
| pubmed-article:21071079 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:21071079 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21071079 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:21071079 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21071079 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21071079 | pubmed:pagination | 1193-203 | lld:pubmed |
| pubmed-article:21071079 | pubmed:dateRevised | 2011-3-11 | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:meshHeading | pubmed-meshheading:21071079... | lld:pubmed |
| pubmed-article:21071079 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21071079 | pubmed:articleTitle | A reducible polycationic gene vector derived from thiolated low molecular weight branched polyethyleneimine linked by 2-iminothiolane. | lld:pubmed |
| pubmed-article:21071079 | pubmed:affiliation | Department of Pharmaceutics and Pharmaceutical Chemistry, The University of Utah, 421 Wakara way, Suite 318, Salt Lake City, UT 84108, USA. | lld:pubmed |
| pubmed-article:21071079 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21071079 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
