Linked Life Data

21058630

Source:http://linkedlifedata.com/resource/pubmed/id/21058630

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pubmed-article:21058630pubmed:abstractTextDipetalogaster maxima is a blood-sucking Hemiptera that inhabits sylvatic areas in Mexico. It usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. Hematophagous insect salivary glands produce potent pharmacologic compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain further insight into the salivary biochemical and pharmacologic complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Salivary proteins were also submitted to one- and two-dimensional gel electrophoresis (1DE and 2DE) followed by mass spectrometry analysis. We present the analysis of a set of 2728 cDNA sequences, 1375 of which coded for proteins of a putative secretory nature. The saliva 2DE proteome displayed approximately 150 spots. The mass spectrometry analysis revealed mainly lipocalins, pallidipins, antigen 5-like proteins, and apyrases. The redundancy of sequence identification of saliva-secreted proteins suggests that proteins are present in multiple isoforms or derive from gene duplications.lld:pubmed
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pubmed-article:21058630pubmed:dateRevised2011-10-6lld:pubmed
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pubmed-article:21058630pubmed:year2011lld:pubmed
pubmed-article:21058630pubmed:articleTitleInsight into the salivary transcriptome and proteome of Dipetalogaster maxima.lld:pubmed
pubmed-article:21058630pubmed:affiliationVector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.lld:pubmed
pubmed-article:21058630pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21058630pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:21058630pubmed:publicationTypeResearch Support, N.I.H., Intramurallld:pubmed