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pubmed-article:2105223pubmed:abstractTextActin filaments in resting discoid bovine platelets were examined by fluorescence and electron microscopy. Rhodamine-phalloidin staining patterns showed a characteristic wheel-like structure which consisted of a central small circle connected by several radial spokes to a large peripheral circle. This wheel-like structure was composed of actin filaments forming a characteristic arrowhead structure with heavy meromyosin from muscle. Actin filaments were densely arrayed in parallel with a marginal microtubule band and radiated out from the center to the periphery. Platelets treated with colchicine lost their marginal microtubule band but retained their wheel-like structure and normal discoid form. Cytochalasin B disrupted the wheel-like structure but not the marginal microtubule band or the normal discoid form. After simultaneous treatment with both cytochalasin B and colchicine, platelets lost their discoid shape. These results suggest that actin filaments and microtubules both play important roles in the maintenance of the discoid shape of resting bovine platelets.lld:pubmed
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pubmed-article:2105223pubmed:articleTitleActin cytoskeleton of resting bovine platelets.lld:pubmed
pubmed-article:2105223pubmed:affiliationDepartment of Biology, Faculty of Science, Osaka University, Japan.lld:pubmed
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pubmed-article:2105223pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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