| pubmed-article:20981424 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C1516213 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C1527169 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0013018 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0079183 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:20981424 | lifeskim:mentions | umls-concept:C0205359 | lld:lifeskim |
| pubmed-article:20981424 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20981424 | pubmed:dateCreated | 2011-1-21 | lld:pubmed |
| pubmed-article:20981424 | pubmed:abstractText | With the aim to identify cyclin B1-derived peptides with high affinity for HLA-A2, we used three in silico prediction algorithms to screen the protein sequence for possible HLA-A2 binders. One peptide scored highest in all three algorithms, and the high HLA-A2-binding affinity of this peptide was verified in an HLA stabilization assay. By stimulation with peptide-loaded dendritic cells a CTL clone was established, which was able to kill two breast cancer cell lines in an HLA-A2-dependent and peptide-specific manner, demonstrating presentation of the peptide on the surface of cancer cells. Furthermore, blood from cancer patients and healthy donors was screened for spontaneous T-cell reactivity against the peptide in IFN-? ELISPOT assays. Patients with breast cancer, malignant melanoma, or renal cell carcinoma hosted powerful and high-frequency T-cell responses against the peptide. In addition, when blood from healthy donors was tested, similar responses were observed. Ultimately, serum from cancer patients and healthy donors was analyzed for anti-cyclin B1 antibodies. Humoral responses against cyclin B1 were frequently detected in both cancer patients and healthy donors. In conclusion, a high-affinity cyclin B1-derived HLA-A2-restricted CTL epitope was identified, which was presented on the cell surface of cancer cells, and elicited spontaneous T-cell responses in cancer patients and healthy donors. | lld:pubmed |
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| pubmed-article:20981424 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20981424 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20981424 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:20981424 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20981424 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:20981424 | pubmed:issn | 1432-0851 | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:BeckerJürgen... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:thor... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:AndersenMads... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:SvaneInge... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:AndersenRikke... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:RitterCathrin... | lld:pubmed |
| pubmed-article:20981424 | pubmed:author | pubmed-author:SørensenRikke... | lld:pubmed |
| pubmed-article:20981424 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20981424 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:20981424 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20981424 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20981424 | pubmed:pagination | 227-34 | lld:pubmed |
| pubmed-article:20981424 | pubmed:dateRevised | 2011-7-25 | lld:pubmed |
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| pubmed-article:20981424 | pubmed:meshHeading | pubmed-meshheading:20981424... | lld:pubmed |
| pubmed-article:20981424 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20981424 | pubmed:articleTitle | Identification of a cyclin B1-derived CTL epitope eliciting spontaneous responses in both cancer patients and healthy donors. | lld:pubmed |
| pubmed-article:20981424 | pubmed:affiliation | Department of Hematology, 54P4, Center for Cancer Immune Therapy, University Hospital Herlev, Herlev Ringvej 75, Herlev, Denmark. risian01@heh.regionh.dk | lld:pubmed |
| pubmed-article:20981424 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20981424 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
