| pubmed-article:20964 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0312402 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0020291 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0022262 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0439857 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0037638 | lld:lifeskim |
| pubmed-article:20964 | lifeskim:mentions | umls-concept:C0599125 | lld:lifeskim |
| pubmed-article:20964 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20964 | pubmed:dateCreated | 1977-12-29 | lld:pubmed |
| pubmed-article:20964 | pubmed:abstractText | The pH dependence of the human prostatic acid phosphatase-catalyzed hydrolysis of p-nitrophenyl phosphate and beta-glyceryl phosphate has been studied over a wide range of pH and the values of Km and V calculated with the aid of the Cleland HYPER program. The pH dependence of Km shows the effect of substrate ionization: pK values of 5.6 and 6.4 are observed as for the respective values of free substrates. The pH dependence of both Km and V for each substrate reveals the involvement of an ionizable group in the ES complex which is ascribed to a phosphohistidine-enzyme intermediate. The small deuterium solvent isotope effects which are observed on V are consistent with values observed for solvolysis of phosphoramidates. The measured data for Km indicates limits on burst-titration experiments of prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2). | lld:pubmed |
| pubmed-article:20964 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20964 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20964 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20964 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:20964 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:20964 | pubmed:author | pubmed-author:Van EttenR... | lld:pubmed |
| pubmed-article:20964 | pubmed:author | pubmed-author:McTigueJ JJJ | lld:pubmed |
| pubmed-article:20964 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:20964 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:20964 | pubmed:volume | 484 | lld:pubmed |
| pubmed-article:20964 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20964 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20964 | pubmed:pagination | 386-97 | lld:pubmed |
| pubmed-article:20964 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:20964 | pubmed:meshHeading | pubmed-meshheading:20964-Hu... | lld:pubmed |
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| pubmed-article:20964 | pubmed:meshHeading | pubmed-meshheading:20964-Bu... | lld:pubmed |
| pubmed-article:20964 | pubmed:meshHeading | pubmed-meshheading:20964-Ki... | lld:pubmed |
| pubmed-article:20964 | pubmed:meshHeading | pubmed-meshheading:20964-Hy... | lld:pubmed |
| pubmed-article:20964 | pubmed:meshHeading | pubmed-meshheading:20964-4-... | lld:pubmed |
| pubmed-article:20964 | pubmed:year | 1977 | lld:pubmed |
| pubmed-article:20964 | pubmed:articleTitle | pH dependence and solvent isotope effects in the hydrolysis of phosphomonoesters by human prostatic acid phosphatase. | lld:pubmed |
| pubmed-article:20964 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20964 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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