| pubmed-article:20937356 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C0205615 | lld:lifeskim |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:20937356 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:20937356 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:20937356 | pubmed:dateCreated | 2010-12-28 | lld:pubmed |
| pubmed-article:20937356 | pubmed:abstractText | GnRH neurons provide the primary driving force upon the neuroendocrine reproductive axis. Here we used GnV-3 cells, a model of conditionally immortalized GnRH-expressing neurons, to perform an analysis of cell cycle and compare the gene expression profile of proliferating cells with differentiated cells. In the proliferation medium, 45 ± 1.5% of GnV-3 cells are in S-phase by FACS analysis. In the differentiation medium, only 9 ± 0.9% of them are in S-phase, and they acquire the characteristic bipolar shape displayed by preoptic GnRH neurons in vivo. In addition, GnV-3 cells in the differentiated state exhibit electrophysiological properties characteristic of neurons. Transcriptomic analysis identified up-regulation of 1931 genes and down-regulation of 1270 genes in cells grown in the differentiation medium compared to cells in the proliferation medium. Subsequent gene ontology study indicated that genes over-expressed in proliferating GnV-3 cells were mainly involved in cell cycle regulations, whereas genes over-expressed in differentiated cells were mainly involved in processes of differentiation, neurogenesis and neuronal morphogenesis. Taken together, these data demonstrate the occurrence of morphological and physiological changes in GnV-3 cells between the proliferating and the differentiated state. Moreover, the genes differentially regulated between these two different states are providing novel pathways potentially important for a better understanding of the physiology of mature GnRH neurons. | lld:pubmed |
| pubmed-article:20937356 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20937356 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20937356 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20937356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20937356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20937356 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20937356 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20937356 | pubmed:issn | 1872-8057 | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:BoccardJulien... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:PralongFranço... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:PrevotVincent... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:PoulainPierre... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:MansuyVirgini... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:ReyJean-Pierr... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:CampagneCélin... | lld:pubmed |
| pubmed-article:20937356 | pubmed:author | pubmed-author:GellerSarahS | lld:pubmed |
| pubmed-article:20937356 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20937356 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20937356 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:20937356 | pubmed:volume | 332 | lld:pubmed |
| pubmed-article:20937356 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20937356 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20937356 | pubmed:pagination | 97-105 | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:meshHeading | pubmed-meshheading:20937356... | lld:pubmed |
| pubmed-article:20937356 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20937356 | pubmed:articleTitle | Phenotypic and molecular characterization of proliferating and differentiated GnRH-expressing GnV-3 cells. | lld:pubmed |
| pubmed-article:20937356 | pubmed:affiliation | Service of Endocrinology, Diabetology and Metabolism, University Hospital and Faculty of Biology and Medicine, 1011 Lausanne, Switzerland. | lld:pubmed |
| pubmed-article:20937356 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20937356 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:25194 | entrezgene:pubmed | pubmed-article:20937356 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20937356 | lld:entrezgene |
