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pubmed-article:2090713pubmed:abstractTextThree fractionation procedures (immunoaffinity chromatography, two-dimensional nondenaturing electrophoresis, and heparin-agarose affinity chromatography) have been compared in determining the kinetics of free and ester cholesterol transfer in normolipemic native plasma. Similar results were obtained in each case. Cell-derived free cholesterol is initially enriched in high density lipoproteins (HDL) (mainly HDL without apoE); at longer time periods (greater than 10 min) greater proportions are observed in very low density lipoproteins (VLDL) and low density lipoproteins (LDL). The major part of cholesteryl ester (about 90%) was retained in HDL, while VLDL and LDL, which contained about 75% of total cholesteryl ester mass, received only about 10% of cell-derived cholesteryl ester. Within HDL, almost all cholesteryl ester was in the apoE-free fraction. These data provide evidence that lipoprotein free and esterified cholesterol are not at chemical equilibrium in normal plasma, and that cell-derived cholesterol is preferentially directed to HDL. The techniques used had a comparable effectiveness for the rapid fractionation of labile lipoprotein lipid radioactivity.lld:pubmed
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pubmed-article:2090713pubmed:authorpubmed-author:FieldingC JCJlld:pubmed
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pubmed-article:2090713pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:2090713pubmed:articleTitleDistribution of cell-derived cholesterol among plasma lipoproteins: a comparison of three techniques.lld:pubmed
pubmed-article:2090713pubmed:affiliationCardiovascular Research Institute, University of California Medical Center, San Francisco, CA 94143.lld:pubmed
pubmed-article:2090713pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2090713pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:2090713pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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