| pubmed-article:20890108 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0007102 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0903042 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0033559 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C1335799 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C1514762 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C1419064 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0086982 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:20890108 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:20890108 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:20890108 | pubmed:dateCreated | 2011-1-5 | lld:pubmed |
| pubmed-article:20890108 | pubmed:abstractText | BACKGROUND: Prostaglandin E? (PGE?) levels are frequently elevated in colorectal carcinomas. PGE? is perceived via four transmembrane G protein coupled receptors (EP1-4), among which the EP4 receptor is most relevant. PGE?/EP4-receptor interaction activates CREB via the ERK/MEK pathway. However, the downstream target genes activated by this pathway remained to be investigated. METHODOLOGY/PRINICIPAL FINDINGS: Here, we have identified S100P (an EF-hand calcium binding protein) as a novel downstream target. We show by realtime RT-PCR that S100P mRNA levels are elevated in 14/17 (82%) colon tumor tissues as compared to paired adjacent normal colonic tissues. S100P expression is stimulated in the presence of PGE? in a time dependent manner at mRNA and protein levels in colon, breast and pancreatic cancer cells. Pharmacological and RNAi-mediated inhibition of the EP4 receptor attenuates PGE?-dependent S100P mRNA induction. RNA(i)-mediated knockdown of CREB inhibits endogenous S100P expression. Furthermore, using luciferase reporter analysis and EMSA we show that mutation and/or deletion of the CRE sequence within the S100P promoter abolished PGE?-mediated transcriptional induction. Finally, we demonstrate that RNA(i)-mediated knockdown of S100P compromised invadopodia formation, colony growth and motility of colon cancer cells. Interestingly, endogenous knock down of S100P decreases ERK expression levels, suggesting a role for ERK in regulating S100P mediated cell growth and motility. CONCLUSIONS/SIGNIFICANCE: Together, our findings show for the first time that S100P expression is regulated by PGE?/EP4-receptor signaling and may participate in a feedback signaling that perpetuates tumor cell growth and migration. Therefore, our data suggest that dysregulated S100P expression resulting from aberrant PGE?/EP4 receptor signaling may have important consequences relevant to colon cancer pathogenesis. | lld:pubmed |
| pubmed-article:20890108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20890108 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20890108 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20890108 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20890108 | pubmed:issn | 1555-8576 | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:ReganJohn WJW | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:NelsonMark... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:LogsdonCraig... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:Mercado-Pimen... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:GibadulinováA... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:PastorekovaSi... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:ArumugamThiru... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:Bhattacharyya... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:OlsonErik RER | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:DickinsonSall... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:ChandramouliA... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:DavenportJenn... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:HutchinsonAnt... | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:ShañasReneéR | lld:pubmed |
| pubmed-article:20890108 | pubmed:author | pubmed-author:OwensJanaeJ | lld:pubmed |
| pubmed-article:20890108 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20890108 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:20890108 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20890108 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20890108 | pubmed:pagination | 1056-66 | lld:pubmed |
| pubmed-article:20890108 | pubmed:dateRevised | 2011-8-1 | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
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| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:meshHeading | pubmed-meshheading:20890108... | lld:pubmed |
| pubmed-article:20890108 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20890108 | pubmed:articleTitle | The induction of S100p expression by the Prostaglandin E? (PGE?)/EP4 receptor signaling pathway in colon cancer cells. | lld:pubmed |
| pubmed-article:20890108 | pubmed:affiliation | Department of Pathology, Arizona Cancer Center, Tucson, USA. | lld:pubmed |
| pubmed-article:20890108 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20890108 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20890108 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:6286 | entrezgene:pubmed | pubmed-article:20890108 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20890108 | lld:entrezgene |
