| pubmed-article:20875052 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0026917 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:20875052 | lifeskim:mentions | umls-concept:C1516044 | lld:lifeskim |
| pubmed-article:20875052 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20875052 | pubmed:dateCreated | 2010-11-9 | lld:pubmed |
| pubmed-article:20875052 | pubmed:abstractText | Macrophage apoptosis represents an important innate defense mechanism against intracellular mycobacterial infection. Previous publications have shown that interferon-? (IFN-?) is involved in apoptosis of immune cells infected with mycobacteria. In this study, the impact of IFN-? treatment on phorbol-12-myristate-13-acetate-differentiated THP-1 cells infected with Mycobacterium bovis was investigated. The results showed that IFN-? increased apoptosis of THP-1 cells infected with M. bovis at a low multiplicity of infection (MOI) in a time-dependent manner. The percentage of cells undergoing apoptosis in IFN-?-treated THP-1 cells increased from 4.3% at 12 h to 36.5% at 72 h upon infection with an MOI of 10. Activation of caspases-3 and -8 increased 8.3- and 6.7-fold, respectively. Neutralizing endogenous tumor necrosis factor-? (TNF-?) significantly inhibited IFN-?-induced apoptosis of M. bovis-infected THP-1 cells. No significant change in IFN-?-induced apoptosis was observed in M. bovis-infected cells after the addition of c-Jun N-terminal kinase and NF-?B pathways' inhibitors. Translocation of apoptosis-inducing factor (AIF) to the nucleus of M. bovis-infected THP-1 cells was observed in 23.4% of IFN-?-treated cells, compared with 11.0% in untreated cells. Taken together, these results suggest that IFN-? promotes apoptosis of M. bovis-infected THP-1 cells during early infection through the TNF-?-mediated death receptor and the AIF apoptotic pathway. | lld:pubmed |
| pubmed-article:20875052 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20875052 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20875052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20875052 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20875052 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:20875052 | pubmed:issn | 1574-695X | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:RoyD SDS | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:WangYangY | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:HuangYingY | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:ZhaoDemingD | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:ZhouXiangmeiX | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:KouadirMohamm... | lld:pubmed |
| pubmed-article:20875052 | pubmed:author | pubmed-author:ZhangJiaoerJ | lld:pubmed |
| pubmed-article:20875052 | pubmed:copyrightInfo | © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20875052 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20875052 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:20875052 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20875052 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20875052 | pubmed:pagination | 191-8 | lld:pubmed |
| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
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| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
| pubmed-article:20875052 | pubmed:meshHeading | pubmed-meshheading:20875052... | lld:pubmed |
| pubmed-article:20875052 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20875052 | pubmed:articleTitle | IFN-? promotes THP-1 cell apoptosis during early infection with Mycobacterium bovis by activating different apoptotic signaling. | lld:pubmed |
| pubmed-article:20875052 | pubmed:affiliation | State Key Lab of Agrobiotechnology, College of Veterinary Medicine, China Agricultural University, Beijing, China. | lld:pubmed |
| pubmed-article:20875052 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20875052 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:3458 | entrezgene:pubmed | pubmed-article:20875052 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20875052 | lld:entrezgene |
