| pubmed-article:20868679 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C0678804 | lld:lifeskim |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C1858460 | lld:lifeskim |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C1419285 | lld:lifeskim |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C0348026 | lld:lifeskim |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:20868679 | lifeskim:mentions | umls-concept:C1261552 | lld:lifeskim |
| pubmed-article:20868679 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20868679 | pubmed:dateCreated | 2010-11-15 | lld:pubmed |
| pubmed-article:20868679 | pubmed:abstractText | We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF1A) and total (GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n=51) or female (n=19) fetus sampled throughout gestation and absent in non-pregnant individuals (n=29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF1A and SRY (?=0.66, P<0.001), total RASSF1A and GLO (?=0.65,P<0.001), SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A ratio (?=0.62, P<0.001) in maternal plasma. The results indicate that a universal fetal marker could be useful not only for the confirmation of the presence of fetal cell-free DNA in maternal plasma but could enable quantification of cell-free fetal DNA in pregnancy associated disorders, independently of the sex of the fetus. | lld:pubmed |
| pubmed-article:20868679 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20868679 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20868679 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20868679 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:20868679 | pubmed:issn | 1096-0945 | lld:pubmed |
| pubmed-article:20868679 | pubmed:author | pubmed-author:DouchaJJ | lld:pubmed |
| pubmed-article:20868679 | pubmed:author | pubmed-author:HromadnikovaI... | lld:pubmed |
| pubmed-article:20868679 | pubmed:author | pubmed-author:ZejskovaLL | lld:pubmed |
| pubmed-article:20868679 | pubmed:author | pubmed-author:JancuskovaTT | lld:pubmed |
| pubmed-article:20868679 | pubmed:author | pubmed-author:KotlabovaKK | lld:pubmed |
| pubmed-article:20868679 | pubmed:copyrightInfo | Copyright © 2010 Elsevier Inc. All rights reserved. | lld:pubmed |
| pubmed-article:20868679 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20868679 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:20868679 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20868679 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20868679 | pubmed:pagination | 241-7 | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
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| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:meshHeading | pubmed-meshheading:20868679... | lld:pubmed |
| pubmed-article:20868679 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20868679 | pubmed:articleTitle | Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma--next step toward reliable non-invasive prenatal diagnostics. | lld:pubmed |
| pubmed-article:20868679 | pubmed:affiliation | Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic. lenka.zej@centrum.cz | lld:pubmed |
| pubmed-article:20868679 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20868679 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
