| pubmed-article:20857263 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0887840 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:20857263 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:20857263 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20857263 | pubmed:dateCreated | 2010-9-21 | lld:pubmed |
| pubmed-article:20857263 | pubmed:abstractText | We recently reported the chromosome region maintenance 1 (CRM1)-dependent nuclear export of intron-less human interferon-?1 (IFN-?1) mRNA, which encodes a main effecter of host innate immunity. We show that the coding region of IFN-?1 mRNA forms novel secondary structures that are responsible for the CRM1-dependent export of the transcript. Deletion-mutagenesis, in vivo export assays, and computer analyses of the folding potentials of export-competent fragments revealed the presence of a domain, termed the conserved secondary structure (CSS), comprising two adjacent putative stable stem-loop structures (nt 208-452). Internal deletion-mutagenesis and constitutive export assays of each stem-loop structure demonstrated that subregions 308-322 and 352-434 act as a core element by conferring the export function on the CSS. Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-?1 mRNA resides within the core element. An RNPS1 (RNA-binding protein S1, serine-rich domain) cDNA was isolated by yeast three-hybrid screening, using bait containing two CSS regions. We showed that RNPS1 might recognize IFN-?1 mRNP that includes CRM1. The data demonstrate that interaction between RNA structures in the coding region and CRM1 affects the nucleocytoplasmic translocation of IFN-?1 mRNA. | lld:pubmed |
| pubmed-article:20857263 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20857263 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20857263 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:20857263 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20857263 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20857263 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20857263 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20857263 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:20857263 | pubmed:issn | 1860-1499 | lld:pubmed |
| pubmed-article:20857263 | pubmed:author | pubmed-author:NishizawaMiki... | lld:pubmed |
| pubmed-article:20857263 | pubmed:author | pubmed-author:ItoSeijiS | lld:pubmed |
| pubmed-article:20857263 | pubmed:author | pubmed-author:YamadaHisaoH | lld:pubmed |
| pubmed-article:20857263 | pubmed:author | pubmed-author:KimuraTominor... | lld:pubmed |
| pubmed-article:20857263 | pubmed:author | pubmed-author:HashimotoIwao... | lld:pubmed |
| pubmed-article:20857263 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20857263 | pubmed:volume | 43 | lld:pubmed |
| pubmed-article:20857263 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20857263 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20857263 | pubmed:pagination | 145-57 | lld:pubmed |
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| pubmed-article:20857263 | pubmed:meshHeading | pubmed-meshheading:20857263... | lld:pubmed |
| pubmed-article:20857263 | pubmed:meshHeading | pubmed-meshheading:20857263... | lld:pubmed |
| pubmed-article:20857263 | pubmed:meshHeading | pubmed-meshheading:20857263... | lld:pubmed |
| pubmed-article:20857263 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20857263 | pubmed:articleTitle | Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-? 1 mRNA. | lld:pubmed |
| pubmed-article:20857263 | pubmed:affiliation | Laboratory of Microbiology and Cell Biology, Department of Pharmacy, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. kimurato@ph.ritsumei.ac.jp | lld:pubmed |
| pubmed-article:20857263 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20857263 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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