| pubmed-article:20850960 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C0036774 | lld:lifeskim |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C1258084 | lld:lifeskim |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:20850960 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:20850960 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20850960 | pubmed:dateCreated | 2010-10-11 | lld:pubmed |
| pubmed-article:20850960 | pubmed:abstractText | A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5-500 ngmL(-1) of BSA-OP, with a detection limit of 0.5 ngmL(-1). It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers. | lld:pubmed |
| pubmed-article:20850960 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20850960 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20850960 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20850960 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20850960 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20850960 | pubmed:issn | 1873-4235 | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:HuF GFG | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:WuHongH | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:WangJunJ | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:LinYueheY | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:ChailapakulOr... | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:LinChiann-Tso... | lld:pubmed |
| pubmed-article:20850960 | pubmed:author | pubmed-author:PinwattanaKul... | lld:pubmed |
| pubmed-article:20850960 | pubmed:copyrightInfo | Copyright © 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20850960 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20850960 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:20850960 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:20850960 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20850960 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20850960 | pubmed:pagination | 1109-13 | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:meshHeading | pubmed-meshheading:20850960... | lld:pubmed |
| pubmed-article:20850960 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20850960 | pubmed:articleTitle | CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin. | lld:pubmed |
| pubmed-article:20850960 | pubmed:affiliation | Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand. | lld:pubmed |
| pubmed-article:20850960 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20850960 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:20850960 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20850960 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:20850960 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
