| pubmed-article:20844583 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20844583 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:20844583 | lifeskim:mentions | umls-concept:C1444748 | lld:lifeskim |
| pubmed-article:20844583 | lifeskim:mentions | umls-concept:C0032149 | lld:lifeskim |
| pubmed-article:20844583 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20844583 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:20844583 | pubmed:dateCreated | 2010-9-16 | lld:pubmed |
| pubmed-article:20844583 | pubmed:abstractText | Several protozoan parasites have been shown to undergo a form of programmed cell death that exhibits morphological features associated with metazoan apoptosis. These include the rodent malaria parasite, Plasmodium berghei. Malaria zygotes develop in the mosquito midgut lumen, forming motile ookinetes. Up to 50% of these exhibit phenotypic markers of apoptosis; as do those grown in culture. We hypothesised that naturally occurring signals induce many ookinetes to undergo apoptosis before midgut traversal. To determine whether nitric oxide and reactive oxygen species act as such triggers, ookinetes were cultured with donors of these molecules. Exposure to the nitric oxide donor SNP induced a significant increase in ookinetes with condensed nuclear chromatin, activated caspase-like molecules and translocation of phosphatidylserine that was dose and time related. Results from an assay that detects the potential-dependent accumulation of aggregates of JC-1 in mitochondria suggested that nitric oxide does not operate via loss of mitochondrial membrane potential. L-DOPA (reactive oxygen species donor) also caused apoptosis in a dose and time dependent manner. Removal of white blood cells significantly decreased ookinetes exhibiting a marker of apoptosis in vitro. Inhibition of the activity of nitric oxide synthase in the mosquito midgut epithelium using L-NAME significantly decreased the proportion of apoptotic ookinetes and increased the number of oocysts that developed. Introduction of a nitric oxide donor into the blood meal had no effect on mosquito longevity but did reduce prevalence and intensity of infection. Thus, nitric oxide and reactive oxygen species are triggers of apoptosis in Plasmodium ookinetes. They occur naturally in the mosquito midgut lumen, sourced from infected blood and mosquito tissue. Up regulation of mosquito nitric oxide synthase activity has potential as a transmission blocking strategy. | lld:pubmed |
| pubmed-article:20844583 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20844583 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20844583 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20844583 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20844583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20844583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20844583 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20844583 | pubmed:issn | 1932-6203 | lld:pubmed |
| pubmed-article:20844583 | pubmed:author | pubmed-author:HurdHilaryH | lld:pubmed |
| pubmed-article:20844583 | pubmed:author | pubmed-author:Al-OlayanEbte... | lld:pubmed |
| pubmed-article:20844583 | pubmed:author | pubmed-author:LewisStevenS | lld:pubmed |
| pubmed-article:20844583 | pubmed:author | pubmed-author:MatthewsHolly... | lld:pubmed |
| pubmed-article:20844583 | pubmed:author | pubmed-author:AliMedhatM | lld:pubmed |
| pubmed-article:20844583 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20844583 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:20844583 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20844583 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:meshHeading | pubmed-meshheading:20844583... | lld:pubmed |
| pubmed-article:20844583 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20844583 | pubmed:articleTitle | Naturally occurring triggers that induce apoptosis-like programmed cell death in Plasmodium berghei ookinetes. | lld:pubmed |
| pubmed-article:20844583 | pubmed:affiliation | School of Life Sciences, Keele University, Keele, United Kingdom. | lld:pubmed |
| pubmed-article:20844583 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20844583 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20844583 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20844583 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20844583 | lld:pubmed |
