pubmed-article:20842623 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0021344 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1704462 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0026724 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0242358 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0029005 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1427865 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0597731 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
pubmed-article:20842623 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
pubmed-article:20842623 | pubmed:issue | 4 | lld:pubmed |
pubmed-article:20842623 | pubmed:dateCreated | 2011-4-7 | lld:pubmed |
pubmed-article:20842623 | pubmed:abstractText | The E6 oncoproteins from high-risk mucosal human papillomavirus (HPV) induce cervical cancer via two major activities, the binding and the degradation of the p53 protein and PDZ domain-containing proteins. Human MAGI-1 is a multi-PDZ domain protein implicated into protein complex assembly at cell-cell contacts. High-risk mucosal HPV E6 proteins interact with the PDZ1 domain of MAGI-1 via a C-terminal consensus binding motif. Here, we developed a medium throughput protocol to accurately measure by surface plasmon resonance affinity constants of protein domains binding to peptidic sequences produced as recombinant fusions to the glutathione-S-transferase (GST). This approach was applied to measure the binding of MAGI-1 PDZ1 to the C-termini of viral or cellular proteins. Both high-risk mucosal HPV E6 C-terminal peptides and cellular partners of MAGI-1 PDZ1 bind to MAGI-1 PDZ1 with comparable dissociation constants in the micromolar range. MAGI-1 PDZ1 shows a preference for C-termini with a valine at position 0 and a negative charge at position -3, confirming previous studies performed with HPV18 E6. A detailed combined analysis via site-directed mutagenesis of the HPV16 C-terminal peptide and PDZ1 indicated that interactions mediated by charged residues upstream the PDZ-binding motif strongly contribute to binding selectivity of this interaction. In addition, our work highlighted the K(499) residue of MAGI-1 as a novel determinant of binding specificity. Finally, we showed that MAGI-1 PDZ1 also binds to the C-termini of LPP and Tax proteins, which were already known to bind to PDZ proteins but not to MAGI-1. | lld:pubmed |
pubmed-article:20842623 | pubmed:language | eng | lld:pubmed |
pubmed-article:20842623 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:20842623 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:20842623 | pubmed:issn | 1099-1352 | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:OrfanoudakisG... | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:KiefferBrunoB | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:NominéYvesY | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:TravéGillesG | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:CharbonnierSe... | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:MassonMuriell... | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:ChapelleAnneA | lld:pubmed |
pubmed-article:20842623 | pubmed:author | pubmed-author:FournaneSadek... | lld:pubmed |
pubmed-article:20842623 | pubmed:copyrightInfo | Copyright © 2010 John Wiley & Sons, Ltd. | lld:pubmed |
pubmed-article:20842623 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:20842623 | pubmed:volume | 24 | lld:pubmed |
pubmed-article:20842623 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:20842623 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:20842623 | pubmed:pagination | 511-23 | lld:pubmed |
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pubmed-article:20842623 | pubmed:articleTitle | Surface plasmon resonance analysis of the binding of high-risk mucosal HPV E6 oncoproteins to the PDZ1 domain of the tight junction protein MAGI-1. | lld:pubmed |
pubmed-article:20842623 | pubmed:affiliation | Équipe Oncoprotéines, FRE 3211, Institut de Recherche de l'École de Biotechnologie de Strasbourg, Université de Strasbourg, Boulevard Sébastien Brandt, BP 10413, 67412 Illkirch Cedex, France. | lld:pubmed |
pubmed-article:20842623 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:20842623 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |