| pubmed-article:20837020 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0023621 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C1801960 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C1540291 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C1710360 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:20837020 | lifeskim:mentions | umls-concept:C1709450 | lld:lifeskim |
| pubmed-article:20837020 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20837020 | pubmed:dateCreated | 2010-12-17 | lld:pubmed |
| pubmed-article:20837020 | pubmed:abstractText | Endosialin/TEM1 is predominantly expressed on neovasculature, thus ideally suited for diagnostic, targeted imaging and therapy of cancer. To isolate TEM1-specific affinity reagents, we thought to screen a recombinant antibody (scFv) library derived from the repertoire of a patient with thrombotic thrombocytopenic purpura (TTP), as autoimmune disorders may produce self-reactive specificities. The yeast-display scFv library was constructed by homologous recombination of the TTP patient repertoire originally expressed on M13 bacteriophage in the novel vector pAGA2 for yeast-display expression. The TTP yeast-display library (10? members) was screened by magnetic and flow sorting with human TEM1 recombinant protein. A pool of yeast-display scFv able to detect 2nM of TEM1 was obtained and transformed into yeast-secreted scFv by homologous recombination using the novel p416 BCCP vector for yeast secretion of biotinylated scFv. Anti-TEM1 yeast-secreted scFv were independently validated in vitro by flow cytometry analysis and ELISA assays, then in vivo biotinylated in N-termini to produce biobodies. Biobody-78 bound specifically to Endosialin/TEM1-expressing ovarian tumor in vivo, with functional stability over 48 h. Our results suggest that our novel paired display-secretory yeast libraries can serve as an ideal platform for the rapid isolation of high-affinity reagents, and that anti-TEM1 biobody-78 can be used for in vitro assays including flow cytometry analysis, as well as in vivo for targeted imaging and therapy of cancer. | lld:pubmed |
| pubmed-article:20837020 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20837020 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20837020 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20837020 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20837020 | pubmed:issn | 1872-7905 | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:SchollerNatha... | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:CoukosGeorgeG | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:SiegelDon LDL | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:LiChunshengC | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:ZhaoAizhiA | lld:pubmed |
| pubmed-article:20837020 | pubmed:author | pubmed-author:Nunez-CruzSel... | lld:pubmed |
| pubmed-article:20837020 | pubmed:copyrightInfo | Copyright © 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20837020 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20837020 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:20837020 | pubmed:volume | 363 | lld:pubmed |
| pubmed-article:20837020 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20837020 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20837020 | pubmed:pagination | 221-32 | lld:pubmed |
| pubmed-article:20837020 | pubmed:dateRevised | 2011-10-5 | lld:pubmed |
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| pubmed-article:20837020 | pubmed:meshHeading | pubmed-meshheading:20837020... | lld:pubmed |
| pubmed-article:20837020 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20837020 | pubmed:articleTitle | Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. | lld:pubmed |
| pubmed-article:20837020 | pubmed:affiliation | Penn Ovarian Cancer Research Center, Center for Research on Reproduction and Women's Health (CRRWH), Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA 19104, USA. | lld:pubmed |
| pubmed-article:20837020 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20837020 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20837020 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
