| pubmed-article:20833635 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0540193 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:20833635 | lifeskim:mentions | umls-concept:C0870509 | lld:lifeskim |
| pubmed-article:20833635 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20833635 | pubmed:dateCreated | 2011-1-24 | lld:pubmed |
| pubmed-article:20833635 | pubmed:abstractText | DNAzymes are easier to prepare and less sensitive to chemical and enzymatic degradation than ribozymes; however, a DNA enzyme expression system has not yet been developed. In this study, we exploited the mechanism of HIV-1 reverse transcription (RT) in a DNA enzyme expression system. We constructed HIV-1 RT-dependent lentiviral DNAzyme expression vectors including the HIV-1 primer binding site, the DNA enzyme, and either a native tRNA (Lys-3), tR(M)DtR(L), or one of two truncated tRNAs (Lys-3), tR(M)D?ARMtR(L) or tR(M)D3'-endtR(L). Lentiviral vector-mediated DNAzyme expression showed high levels of inhibition of HIV-1 replication in SupT1 cells. We also demonstrated the usefulness of this approach in a long-term assay, in which we found that the DNAzymes prevented escape from inhibition of HIV. These results suggest that HIV-1 RT-dependent lentiviral vector-derived DNAzymes prevent the emergence of escape mutations. | lld:pubmed |
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| pubmed-article:20833635 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20833635 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20833635 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20833635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20833635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20833635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20833635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20833635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20833635 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20833635 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20833635 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:20833635 | pubmed:author | pubmed-author:YamamotoNorio... | lld:pubmed |
| pubmed-article:20833635 | pubmed:author | pubmed-author:TakakuHiroshi... | lld:pubmed |
| pubmed-article:20833635 | pubmed:author | pubmed-author:HayafuneMasaa... | lld:pubmed |
| pubmed-article:20833635 | pubmed:author | pubmed-author:HabuYuichiroY | lld:pubmed |
| pubmed-article:20833635 | pubmed:author | pubmed-author:SugiyamaRyuic... | lld:pubmed |
| pubmed-article:20833635 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20833635 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:20833635 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20833635 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20833635 | pubmed:pagination | 589-98 | lld:pubmed |
| pubmed-article:20833635 | pubmed:dateRevised | 2011-7-20 | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
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| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:meshHeading | pubmed-meshheading:20833635... | lld:pubmed |
| pubmed-article:20833635 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20833635 | pubmed:articleTitle | HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses. | lld:pubmed |
| pubmed-article:20833635 | pubmed:affiliation | Department of Life and Environmental Science, Chiba Institute of Technology, Narashino-shi, Chiba, Japan. | lld:pubmed |
| pubmed-article:20833635 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20833635 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
