| pubmed-article:20833546 | pubmed:abstractText | Carbonic anhydrases (CAs, EC 4.2.1.1) belonging to ?-, ?-, ?- and ?-classes and from various organisms, ranging from the bacteria, archaea to eukarya domains, were investigated for their esterase/phosphatase activity with 4-nitrophenyl acetate, 4-nitrophenyl phosphate and paraoxon as substrates. Only ?-CAs showed esterase/phosphatase activity, whereas enzymes belonging to the ?-, ?- and ?-classes were completely devoid of such activity. Paraoxon, the metabolite of the organophosphorus insecticide parathione, was a much better substrate for several human/murine ?-CA isoforms (CA I, II and XIII), with k(cat)/K(M) in the range of 2681.6-4474.9M(-1)s(-1), compared to 4-nitrophenyl phosphate (k(cat)/K(M) of 14.9-1374.4M(-1)s(-1)). | lld:pubmed |
