| pubmed-article:20814979 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0025552 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0012727 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C1704241 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0597526 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0302891 | lld:lifeskim |
| pubmed-article:20814979 | lifeskim:mentions | umls-concept:C0348080 | lld:lifeskim |
| pubmed-article:20814979 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:20814979 | pubmed:dateCreated | 2010-9-3 | lld:pubmed |
| pubmed-article:20814979 | pubmed:abstractText | Increases in the study of protein-metal complexes, as well as in metal displacement in protein-metal complexes under native conditions for optimum catalytic properties in drug research and catalyst design, demands a separation/detection technology that can accurately measure metal displacement and stoichiometry in protein-metal complexes. Both nuclear magnetic resonance (NMR) and X-ray diffraction techniques have been used for this purpose; however, these techniques lack sensitivity. Electrospray ionization mass spectrometry (ESI-MS) using direct infusion offers higher sensitivity than the former techniques and provides molecular distribution of various protein-metal complexes. However, since protein-metal complexes under native conditions usually are dissolved in salt solutions, their direct ESI-MS analysis requires off-line sample clean-up prior to MS analysis to avoid sample suppression during ESI. Moreover, direct infusion of the salty solution promotes non-specific salt adduct formation by the protein-metal complexes under ESI-MS, which complicates the identification and stoichiometry measurements of the protein-metal complexes. Because of the high mass of protein-metal complexes and lack of sufficient resolution by most mass spectrometers to separate non-specific from specific metal-protein complexes, accurate protein-metal stoichiometry measurements require some form of sample clean up prior to ESI-MS analysis. In this study, we demonstrate that capillary electrophoresis/electrospray ionization in conjunction with a medium-resolution (approximately 10,000) mass spectrometer is an efficient and fast method for the measurement of the stoichiometry of the protein-metal complexes under physiological conditions (pH approximately 7). The metal displacement of Co(2+) to Cd(2+), two metal ions necessary for activation in the monomeric AHL lactonase produced by B. thuringiensis, has been used as a proof of concept. | lld:pubmed |
| pubmed-article:20814979 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20814979 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20814979 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20814979 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:20814979 | pubmed:issn | 1097-0231 | lld:pubmed |
| pubmed-article:20814979 | pubmed:author | pubmed-author:MoiniMehdiM | lld:pubmed |
| pubmed-article:20814979 | pubmed:author | pubmed-author:FastWalterW | lld:pubmed |
| pubmed-article:20814979 | pubmed:author | pubmed-author:ThomasPei WPW | lld:pubmed |
| pubmed-article:20814979 | pubmed:author | pubmed-author:GarzaSelyndaS | lld:pubmed |
| pubmed-article:20814979 | pubmed:copyrightInfo | 2010 John Wiley & Sons, Ltd. | lld:pubmed |
| pubmed-article:20814979 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20814979 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:20814979 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20814979 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20814979 | pubmed:pagination | 2730-4 | lld:pubmed |
| pubmed-article:20814979 | pubmed:dateRevised | 2011-6-6 | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:meshHeading | pubmed-meshheading:20814979... | lld:pubmed |
| pubmed-article:20814979 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20814979 | pubmed:articleTitle | Metal displacement and stoichiometry of protein-metal complexes under native conditions using capillary electrophoresis/mass spectrometry. | lld:pubmed |
| pubmed-article:20814979 | pubmed:affiliation | Cedra Corp., Austin, TX, USA. [corrected] | lld:pubmed |
| pubmed-article:20814979 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20814979 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
