| pubmed-article:2078556 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2078556 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:2078556 | lifeskim:mentions | umls-concept:C1883178 | lld:lifeskim |
| pubmed-article:2078556 | lifeskim:mentions | umls-concept:C0014139 | lld:lifeskim |
| pubmed-article:2078556 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2078556 | pubmed:dateCreated | 1991-5-1 | lld:pubmed |
| pubmed-article:2078556 | pubmed:abstractText | Subclones of the HT29-18 clone, derived from a human adenocarcinoma, are able to acquire an enterocyte-like phenotype depending on the culture conditions. To investigate fluid-phase and receptor-mediated endocytosis in the polarized subclone HT29-18-C1, we established culture conditions that allowed cell growth on permeable supports. HT29-18-C1 monolayers had an electrical resistance of 43 ohms.cm2 and developed a transepithelial potential of about 2 mV. Transferrin receptors were uniformly distributed on the entire cell surface of undifferentiated HT29-18 cells but were located on the basolateral membrane of differentiated cells. Transferrin had a high affinity (Kd = 2.5 x 10(-9) M) for its receptor independent of the state of differentiation. The number of transferrin receptors and the mRNA amounts encoding them were comparable in the undifferentiated and differentiated HT29-18 cells. Transferrin was quickly internalized and recycled back to the cell surface of undifferentiated HT29-18 cells. The same phenomenon also occurred in differentiated HT29-18 cells, but the receptors were limited to the basolateral membrane. In the presence of ammonium chloride, the process was slower but remained polarized. Fluid-phase uptake was also investigated with horseradish peroxidase (HRP) in differentiated HT29-18 C1 cells. HRP that was internalized in 1 hour from a given membrane domain preferentially recycled back to the same membrane domain. No significant accumulation of the enzyme in the late endosomes and lysosomes of the differentiated HT29-18-C1 cells was observed. | lld:pubmed |
| pubmed-article:2078556 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2078556 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2078556 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2078556 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2078556 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2078556 | pubmed:issn | 1043-4674 | lld:pubmed |
| pubmed-article:2078556 | pubmed:author | pubmed-author:Dautry-Varsat... | lld:pubmed |
| pubmed-article:2078556 | pubmed:author | pubmed-author:LouvardDD | lld:pubmed |
| pubmed-article:2078556 | pubmed:author | pubmed-author:HuetCC | lld:pubmed |
| pubmed-article:2078556 | pubmed:author | pubmed-author:GodefroyOO | lld:pubmed |
| pubmed-article:2078556 | pubmed:author | pubmed-author:IbarraCC | lld:pubmed |
| pubmed-article:2078556 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2078556 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:2078556 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2078556 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2078556 | pubmed:pagination | 875-86 | lld:pubmed |
| pubmed-article:2078556 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:meshHeading | pubmed-meshheading:2078556-... | lld:pubmed |
| pubmed-article:2078556 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2078556 | pubmed:articleTitle | Establishment of polarized endocytosis in differentiable intestinal HT29-18 subclones. | lld:pubmed |
| pubmed-article:2078556 | pubmed:affiliation | Département de Biologie Moléculaire, URA CNRS 1149, Institut Pasteur, Paris, France. | lld:pubmed |
| pubmed-article:2078556 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2078556 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2078556 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2078556 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2078556 | lld:pubmed |
